• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过小分子筛选发现 CHIR99021 和 Brdu 对鸡 iPSC 重编程非常关键。

CHIR99021 and Brdu Are Critical in Chicken iPSC Reprogramming via Small-Molecule Screening.

机构信息

Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.

Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

出版信息

Genes (Basel). 2024 Sep 13;15(9):1206. doi: 10.3390/genes15091206.

DOI:10.3390/genes15091206
PMID:39336797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11431361/
Abstract

Induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells into cells with most of the ESC (embryonic stem cell) characteristics show promise toward solving ethical problems currently facing stem cell research and eventually yield clinical grade pluripotent stem cells for therapies and regenerative medicine. In recent years, an increasing body of research suggests that the chemical induction of pluripotency (CIP) method can yield iPSCs in vitro, yet its application in avian species remains unreported. Herein, we successfully obtained stably growing chicken embryonic fibroblasts (CEFs) using the tissue block adherence method and employed 12 small-molecule compounds to induce chicken iPSC formation. The final optimized iPSC induction system was bFGF (10 ng/mL), CHIR99021 (3 μM), RepSox (5 μM), DZNep (0.05 μM), BrdU (10 μM), BMP4 (10 ng/mL), vitamin C (50 μg/mL), EPZ-5676 (5 μM), and VPA (0.1 mM). Optimization of the induction system revealed that the highest number of clones was induced with 8 × 10 cells per well and at 1.5 times the original concentration. Upon characterization, these clones exhibited iPSC characteristics, leading to the development of a stable compound combination for iPSC generation in chickens. Concurrently, employing a deletion strategy to investigate the functionality of small-molecule compounds during induction, we identified CHIR99021 and BrdU as critical factors for inducing chicken iPSC formation. In conclusion, this study provides a reference method for utilizing small-molecule combinations in avian species to reprogram cells and establish a network of cell fate determination mechanisms.

摘要

诱导多能干细胞（iPSCs）是由体细胞重编程为具有多数胚胎干细胞（ESC）特征的细胞，有望解决当前干细胞研究面临的伦理问题，并最终为治疗和再生医学提供临床级别的多能干细胞。近年来，越来越多的研究表明，化学诱导多能性（CIP）方法可以在体外产生 iPSCs，但其在禽类中的应用尚未报道。在此，我们成功地使用组织块贴壁法获得了稳定生长的鸡胚胎成纤维细胞（CEFs），并采用 12 种小分子化合物诱导鸡 iPSC 的形成。最终优化的 iPSC 诱导系统为 bFGF（10ng/mL）、CHIR99021（3μM）、RepSox（5μM）、DZNep（0.05μM）、BrdU（10μM）、BMP4（10ng/mL）、维生素 C（50μg/mL）、EPZ-5676（5μM）和 VPA（0.1mM）。诱导系统的优化表明，在每孔 8×10 个细胞和原始浓度的 1.5 倍条件下，诱导克隆数最高。这些克隆表现出 iPSC 特征，表明建立了一种稳定的化合物组合，可用于在鸡中生成 iPSC。同时，通过采用缺失策略研究小分子化合物在诱导过程中的功能，我们确定 CHIR99021 和 BrdU 是诱导鸡 iPSC 形成的关键因素。总之，本研究为利用小分子组合在禽类中重编程细胞并建立细胞命运决定机制网络提供了参考方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/a0ca1c49505a/genes-15-01206-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/7b4aa64accf7/genes-15-01206-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/9fc502f5ebf1/genes-15-01206-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/8fc04b027403/genes-15-01206-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/9256ab7df545/genes-15-01206-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/99b476f4663d/genes-15-01206-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/0e82961f58ca/genes-15-01206-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/5d7db3182d49/genes-15-01206-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/a0ca1c49505a/genes-15-01206-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/7b4aa64accf7/genes-15-01206-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/9fc502f5ebf1/genes-15-01206-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/8fc04b027403/genes-15-01206-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/9256ab7df545/genes-15-01206-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/99b476f4663d/genes-15-01206-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/0e82961f58ca/genes-15-01206-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/5d7db3182d49/genes-15-01206-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5764/11431361/a0ca1c49505a/genes-15-01206-g008.jpg

相似文献

1
CHIR99021 and Brdu Are Critical in Chicken iPSC Reprogramming via Small-Molecule Screening.通过小分子筛选发现 CHIR99021 和 Brdu 对鸡 iPSC 重编程非常关键。
Genes (Basel). 2024 Sep 13;15(9):1206. doi: 10.3390/genes15091206.
2
Reprogramming of chimpanzee fibroblasts into a multipotent cancerous but not fully pluripotent state by transducing iPSC factors in 2i/LIF culture.在 2i/LIF 培养体系中转导 iPSC 因子将食蟹猴成纤维细胞重编程为具有多能性的癌性细胞，但不是完全多能性。
Differentiation. 2020 Mar-Apr;112:67-76. doi: 10.1016/j.diff.2020.01.002. Epub 2020 Feb 4.
3
Mechanism of Induction: Induced Pluripotent Stem Cells (iPSCs).诱导机制：诱导多能干细胞（iPSCs）。
J Stem Cells. 2015;10(1):43-62.
4
Transient folate deprivation in combination with small-molecule compounds facilitates the generation of somatic cell-derived pluripotent stem cells in mice.短暂叶酸缺乏与小分子化合物相结合可促进小鼠体细胞来源的多能干细胞的产生。
J Huazhong Univ Sci Technolog Med Sci. 2014 Apr;34(2):151-156. doi: 10.1007/s11596-014-1249-5. Epub 2014 Apr 8.
5
Controlling the Switch from Neurogenesis to Pluripotency during Marmoset Monkey Somatic Cell Reprogramming with Self-Replicating mRNAs and Small Molecules.利用自我复制的 mRNA 和小分子控制狨猴体细胞重编程中神经发生到多能性的转变。
Cells. 2020 Nov 5;9(11):2422. doi: 10.3390/cells9112422.
6
Small Molecules that Promote Self-Renewal of Stem Cells and Somatic Cell Reprogramming.促进干细胞自我更新和体细胞重编程的小分子。
Stem Cell Rev Rep. 2020 Jun;16(3):511-523. doi: 10.1007/s12015-020-09965-w.
7
5-Aza-2'-Deoxycytidine and Valproic Acid in Combination with CHIR99021 and A83-01 Induce Pluripotency Genes Expression in Human Adult Somatic Cells.5-氮杂-2'-脱氧胞苷和丙戊酸联合 CHIR99021 和 A83-01 诱导人成体细胞中多能性基因的表达。
Molecules. 2021 Mar 29;26(7):1909. doi: 10.3390/molecules26071909.
8
Generation of intermediate porcine iPS cells under culture condition favorable for mesenchymal-to-epithelial transition.在有利于间充质向上皮细胞转化的培养条件下生成中间型猪诱导多能干细胞。
Stem Cell Rev Rep. 2015 Feb;11(1):24-38. doi: 10.1007/s12015-014-9552-x.
9
Optimized Approaches for Generation of Integration-free iPSCs from Human Urine-Derived Cells with Small Molecules and Autologous Feeder.优化方法：从小分子和自体饲养细胞中生成无整合人诱导多能干细胞。
Stem Cell Reports. 2016 May 10;6(5):717-728. doi: 10.1016/j.stemcr.2016.04.001. Epub 2016 Apr 28.
10
The small molecule inhibitors PD0325091 and CHIR99021 reduce expression of pluripotency-related genes in putative porcine induced pluripotent stem cells.小分子抑制剂PD0325091和CHIR99021可降低猪诱导多能干细胞中多能性相关基因的表达。
Cell Reprogram. 2014 Aug;16(4):235-40. doi: 10.1089/cell.2014.0010. Epub 2014 Jun 24.

本文引用的文献

1
The Effect of CHIR 99021, a Glycogen Synthase Kinase-3β Inhibitor, on Transforming Growth Factor β-Induced Tenon Fibrosis.CHIR 99021，一种糖原合成酶激酶-3β抑制剂，对转化生长因子-β诱导的腱膜纤维化的影响。
Invest Ophthalmol Vis Sci. 2021 Dec 1;62(15):25. doi: 10.1167/iovs.62.15.25.
2
Small Molecules that Promote Self-Renewal of Stem Cells and Somatic Cell Reprogramming.促进干细胞自我更新和体细胞重编程的小分子。
Stem Cell Rev Rep. 2020 Jun;16(3):511-523. doi: 10.1007/s12015-020-09965-w.
3
Chromatin Accessibility Dynamics during Chemical Induction of Pluripotency.
化学诱导多能性过程中染色质可及性动态变化。
Cell Stem Cell. 2018 Apr 5;22(4):529-542.e5. doi: 10.1016/j.stem.2018.03.005.
4
Technical note: Induction of pluripotent stem cell-like cells from chicken feather follicle cells.技术说明：从鸡毛毛囊细胞诱导多能干细胞样细胞。
J Anim Sci. 2017 Aug;95(8):3479-3486. doi: 10.2527/jas.2017.1418.
5
A XEN-like State Bridges Somatic Cells to Pluripotency during Chemical Reprogramming.XEN 样状态在化学重编程过程中连接体细胞核细胞到多能性。
Cell. 2015 Dec 17;163(7):1678-91. doi: 10.1016/j.cell.2015.11.017. Epub 2015 Dec 10.
6
Human embryonic stem cells.人类胚胎干细胞。
Best Pract Res Clin Obstet Gynaecol. 2016 Feb;31:2-12. doi: 10.1016/j.bpobgyn.2015.08.010. Epub 2015 Sep 11.
7
Bromodeoxyuridine promotes full-chemical induction of mouse pluripotent stem cells.溴脱氧尿苷促进小鼠多能干细胞的完全化学诱导。
Cell Res. 2015 Oct;25(10):1171-4. doi: 10.1038/cr.2015.96. Epub 2015 Aug 7.
8
Molecular basis of embryonic stem cell self-renewal: from signaling pathways to pluripotency network.胚胎干细胞自我更新的分子基础：从信号通路到多能性网络
Cell Mol Life Sci. 2015 May;72(9):1741-57. doi: 10.1007/s00018-015-1833-2. Epub 2015 Jan 17.
9
The generation of definitive endoderm from human embryonic stem cells is initially independent from activin A but requires canonical Wnt-signaling.人胚胎干细胞向 definitive endoderm 的分化最初不依赖于 activin A，但需要经典的 Wnt 信号通路。
Stem Cell Rev Rep. 2014 Aug;10(4):480-93. doi: 10.1007/s12015-014-9509-0.
10
Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds.小分子化合物诱导的小鼠体细胞多能干细胞。
Science. 2013 Aug 9;341(6146):651-4. doi: 10.1126/science.1239278. Epub 2013 Jul 18.