Kim Y M, Park Y H, Lim J M, Jung H, Han J Y
J Anim Sci. 2017 Aug;95(8):3479-3486. doi: 10.2527/jas.2017.1418.
Pluripotent stem cells including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) are regarded as representative tools for conservation of animal genetic resources. Although ESC have been established from chicken, it is very difficult to obtain enough embryos for isolation of stem cells for avian conservation in most wild birds. Therefore, the high feasibility of obtaining the pluripotent cell is most important in avian conservation studies. In this study, we generated induced pluripotent stem cell-like cells (iPSLC) from avian Feather Follicular cells (FFC). Avian FFC are one of the most easily accessible cell sources in most avian species, and their reprogramming into pluripotent stem cells can be an alternative system for preservation of avian species. Intriguingly, FFC had mesenchymal stromal cells (MSC)-like characteristics with regard to gene expression, protein expression, and adipocyte differentiation. Subsequently, we attempted to generate iPSLC from FFC using retroviral vectors. The FFC-iPSLC can proliferate with the stem pluripotent property and differentiate into several types of cells in vitro. Our results suggest that chicken FFC are an alternative cell source for avian cell reprogramming into pluripotent stem cells. This experimental strategy should be useful for conservation and restoration of endangered or high-value avian species without sacrificing embryos.
包括胚胎干细胞（ESC）和诱导多能干细胞（iPSC）在内的多能干细胞被视为保护动物遗传资源的代表性工具。虽然已经从鸡中建立了胚胎干细胞，但在大多数野生鸟类中，很难获得足够的胚胎来分离用于鸟类保护的干细胞。因此，获得多能细胞的高可行性在鸟类保护研究中最为重要。在本研究中，我们从鸟类毛囊细胞（FFC）中生成了诱导多能干细胞样细胞（iPSLC）。鸟类毛囊细胞是大多数鸟类中最容易获取的细胞来源之一，将它们重编程为多能干细胞可以成为保护鸟类物种的另一种系统。有趣的是，毛囊细胞在基因表达、蛋白质表达和脂肪细胞分化方面具有间充质基质细胞（MSC）样的特征。随后，我们尝试使用逆转录病毒载体从毛囊细胞中生成诱导多能干细胞样细胞。毛囊细胞来源的诱导多能干细胞样细胞可以凭借干细胞多能特性进行增殖，并在体外分化为几种类型的细胞。我们的结果表明，鸡的毛囊细胞是将鸟类细胞重编程为多能干细胞的另一种细胞来源。这种实验策略对于保护和恢复濒危或高价值鸟类物种而不牺牲胚胎应该是有用的。
