School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, China.
Research Center for Stress Physiology in Fruit Trees, Hebei University of Engineering, Handan 056038, China.
Genes (Basel). 2024 Sep 23;15(9):1235. doi: 10.3390/genes15091235.
BACKGROUND/OBJECTIVES: Nitrogen is an essential macroelement for plant growth and productivity. Calcium (Ca) acts as a critical second messenger in numerous adaptations and developmental processes in plants. The Calcineurin B-like protein (CBL)-interacting protein kinase (CIPK) signaling pathway has been demonstrated to be involved in multiple intracellular ion homeostasis of plants in response to stresses. However, whether CIPKs are involved in nitrate deficiency stress remains largely unknown.
In this study, we screened T-DNA insertion mutants of the CIPK family under nitrate deficiency conditions by a reverse genetic strategy.
We found that the mutant showed a shorter primary root and had a lower fresh weight and total N content compared with wildtype (WT) plants under nitrate deficiency. The complementation lines completely rescued the sensitive phenotype. Additionally, mutation caused nitrogen-starvation marker genes to be decreased under nitrate deficiency. We further found that CIPK1 interacted with teosintebranched 1/cycloidea/proliferating cell factor 1-20 (TCP20) in a yeast two-hybrid system.
Collectively, our results reveal a novel role of CIPK1 in response to nitrate deficiency in .
背景/目的：氮是植物生长和生产力的必需宏量元素。钙（Ca）在植物的许多适应和发育过程中充当关键的第二信使。钙调神经磷酸酶 B 样蛋白（CBL）-相互作用蛋白激酶（CIPK）信号通路已被证明参与植物对胁迫的多种细胞内离子稳态。然而，CIPKs 是否参与硝酸盐缺乏胁迫在很大程度上仍不清楚。
本研究通过反向遗传学策略筛选硝酸盐缺乏条件下 CIPK 家族的 T-DNA 插入突变体。
我们发现与野生型（WT）植物相比，突变体在硝酸盐缺乏条件下表现出较短的主根，鲜重和总氮含量较低。 互补系完全挽救了敏感表型。此外， 突变导致氮饥饿标记基因在硝酸盐缺乏时减少。我们进一步发现 CIPK1 在酵母双杂交系统中与蜀黍分支 1/环化/增殖细胞因子 1-20（TCP20）相互作用。
总之，我们的结果揭示了 CIPK1 在 响应硝酸盐缺乏中的新作用。
