环状E3连接酶BOI与EXO70E2相互作用并介导其在……中的泛素化。
The RING-Type E3 Ligase BOI Interacts with EXO70E2 and Mediates Its Ubiquitination in .
作者信息
Li Zhaowu, Huang Jianzhong, Hu Yue, Zhou Xiaojie, Tan Xiao, Wang Zhangying, Gao Zhiyong, Wu Xiaoqiu
机构信息
Puai Medical College, Shaoyang University, Shaoyang 422000, China.
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
出版信息
Life (Basel). 2024 Sep 17;14(9):1169. doi: 10.3390/life14091169.
The exocyst is a hetero-octameric complex that exhibits significant functional diversity in regulating biological processes and defense responses. In plants, the EXO70 proteins are important components of the exocyst complex and are involved in membrane trafficking, biotic and abiotic interactions, as well as cell wall formation. A previous study has indicated that a member of the EXO subfamily, EXO70E2, interacts with RIN4 to mediate plant immunity. In this study, we found that EXO70E2 interacts with the RING-type E3 ligase Botrytis susceptible1 interactor (BOI), and the C-terminal domain of BOI is necessary for its interaction with EXO70E2. Moreover, the protein level of EXO70E2 was degraded and ubiquitinated by BOI in vitro. Collectively, our study reveals a mechanism for regulating the stability of EXO70E2 by a RING-type E3 ligase BOI-mediated ubiquitination.
外泌体是一种异源八聚体复合物，在调节生物过程和防御反应方面表现出显著的功能多样性。在植物中，EXO70蛋白是外泌体复合物的重要组成部分，参与膜运输、生物和非生物相互作用以及细胞壁形成。先前的一项研究表明，EXO亚家族的一个成员EXO70E2与RIN4相互作用以介导植物免疫。在本研究中，我们发现EXO70E2与RING型E3连接酶灰霉病易感1相互作用蛋白（BOI）相互作用，并且BOI的C末端结构域是其与EXO70E2相互作用所必需的。此外，EXO70E2的蛋白质水平在体外被BOI降解并泛素化。总的来说，我们的研究揭示了一种通过RING型E3连接酶BOI介导的泛素化来调节EXO70E2稳定性的机制。
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