Bertorello Sara, Cei Francesco, Fink Dorian, Niccolai Elena, Amedei Amedeo
Department of Experimental and Clinical Medicine, University of Florence, 50139 Florence, Italy.
Laboratorio Congiunto MIA-LAB (Microbiome-Immunity Axis Research for a Circular Health), University of Florence, 50134 Florence, Italy.
Microorganisms. 2024 Sep 4;12(9):1828. doi: 10.3390/microorganisms12091828.
Investigating the complex interactions between microbiota and immunity is crucial for a fruitful understanding progress of human health and disease. This review assesses animal models, next-generation in vitro models, and in silico approaches that are used to decipher the microbiome-immunity axis, evaluating their strengths and limitations. While animal models provide a comprehensive biological context, they also raise ethical and practical concerns. Conversely, modern in vitro models reduce animal involvement but require specific costs and materials. When considering the environmental impact of these models, in silico approaches emerge as promising for resource reduction, but they require robust experimental validation and ongoing refinement. Their potential is significant, paving the way for a more sustainable and ethical future in microbiome-immunity research.
研究微生物群与免疫系统之间的复杂相互作用对于卓有成效地理解人类健康与疾病的进展至关重要。本综述评估了用于解读微生物组 - 免疫轴的动物模型、新一代体外模型和计算机方法，评估了它们的优势和局限性。虽然动物模型提供了全面的生物学背景，但也引发了伦理和实际问题。相反，现代体外模型减少了动物的使用，但需要特定的成本和材料。在考虑这些模型的环境影响时，计算机方法在减少资源方面显示出前景，但它们需要强有力的实验验证和持续改进。它们的潜力巨大，为微生物组 - 免疫研究更具可持续性和伦理的未来铺平了道路。
