Ngwe Tun Mya Myat, Kapandji Merveille, Wada Atsuhiko, Yamamoto Ko, Dumre Shyam Prakash, Nwe Khine Mya, Lin Htin, Takamatsu Yuki, Thant Kyaw Zin, Thu Hlaing Myat, Urano Takeshi, Pandey Basu Dev, Morita Kouichi
Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
Pathogens. 2024 Sep 23;13(9):818. doi: 10.3390/pathogens13090818.
Dengue is a viral infection caused by the dengue virus (DENV), transmitted to humans through the bite of infected mosquitoes. About half of the world's population is now at risk of dengue, which represents a global public health concern, especially in tropical and subtropical countries. Early detection of the viral infection is crucial to manage the disease; hence, effective rapid diagnostic tests are essential. In this study, we evaluated the performance between the new Fujifilm Dengue non-structural antigen diagnosis kit (FF NS1 kit) and the SD Bioline NS1 antigen test kit (SD NS1 kit) against the quantitative real-time polymerase chain reaction (qRT-PCR) assays. The 140 acute serum samples collected from the Yangon General Hospital and Yangon Children's Hospital, Myanmar, from 2017 to 2019 were characterised by the three assays. With the qRT-PCR as the standard, the FF NS1 kit and the SD NS1 kit exhibited sensitivity of 94.3% and 88.6%, respectively, and specificity of 100% in both kits. Moreover, the positivity rates of the FF NS1 kit and the SD NS1 kit were 97.5% and 95% in primary infection and 90% and 80% in secondary infection, respectively. Our overall results suggest that the FF NS1 kit is reliable and accurate for detecting DENV infection.
登革热是一种由登革病毒（DENV）引起的病毒感染，通过受感染蚊子的叮咬传播给人类。目前，全球约一半人口面临登革热风险，这是一个全球公共卫生问题，在热带和亚热带国家尤为突出。早期检测病毒感染对于控制该疾病至关重要；因此，有效的快速诊断测试必不可少。在本研究中，我们针对定量实时聚合酶链反应（qRT-PCR）检测，评估了新型富士胶片登革热非结构抗原诊断试剂盒（FF NS1试剂盒）和SD生物线NS1抗原检测试剂盒（SD NS1试剂盒）的性能。从缅甸仰光总医院和仰光儿童医院收集的140份2017年至2019年的急性血清样本，通过这三种检测进行了特征分析。以qRT-PCR为标准，FF NS1试剂盒和SD NS1试剂盒的敏感性分别为94.3%和88.6%，两种试剂盒的特异性均为100%。此外，FF NS1试剂盒和SD NS1试剂盒在初次感染中的阳性率分别为97.5%和95%，在二次感染中的阳性率分别为90%和80%。我们的总体结果表明，FF NS1试剂盒在检测DENV感染方面可靠且准确。
