Zhang Pengcheng, Wang Jingjing, Yang Yajie, Pan Jingjing, Bai Xuelian, Zhou Ting, Lai Tongfei
College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China.
Plants (Basel). 2024 Sep 21;13(18):2650. doi: 10.3390/plants13182650.
Tomato fruit ripening is an elaborate genetic trait correlating with significant changes at physiological and biochemical levels. Sugar metabolism plays an important role in this highly orchestrated process and ultimately determines the quality and nutritional value of fruit. However, the mode of molecular regulation is not well understood. Galactinoal-sucrose galactosyltransferase (GSGT), a key enzyme in the biosynthesis of raffinose family oligosaccharides (RFOs), can transfer the galactose unit from 1-α-D-galactosyl-myo-inositol to sucrose and yield raffinose, or catalyze the reverse reaction. In the present study, the expression of was decreased by Potato Virus X (PVX)-mediated gene silencing, which led to an unripe phenotype in tomato fruit. The physiological and biochemical changes induced by silencing suggested that the process of fruit ripening was delayed as well. silencing also led to significant changes in gene expression levels associated with ethylene production, pigment accumulation, and ripening-associated transcription factors (TFs). In addition, the interaction between SlGSGT2 and SlSPL-CNR indicated a possible regulatory mechanism via ripening-related TFs. These findings would contribute to illustrating the biological functions of in tomato fruit ripening and quality forming.
番茄果实成熟是一种复杂的遗传特性，与生理和生化水平的显著变化相关。糖代谢在这个高度协调的过程中起着重要作用，并最终决定果实的品质和营养价值。然而，分子调控模式尚未得到很好的理解。半乳糖基 - 蔗糖半乳糖基转移酶（GSGT）是棉子糖家族寡糖（RFOs）生物合成中的关键酶，它可以将半乳糖单元从1-α-D-半乳糖基 - 肌醇转移到蔗糖上生成棉子糖，或催化逆反应。在本研究中，通过马铃薯X病毒（PVX）介导的基因沉默降低了（此处原文缺失具体基因名称）的表达，这导致番茄果实出现未成熟表型。（此处原文缺失具体基因名称）沉默诱导的生理和生化变化表明果实成熟过程也被延迟。（此处原文缺失具体基因名称）沉默还导致与乙烯产生、色素积累和成熟相关转录因子（TFs）相关的基因表达水平发生显著变化。此外，SlGSGT2与SlSPL-CNR之间的相互作用表明可能存在通过成熟相关TFs的调控机制。这些发现将有助于阐明（此处原文缺失具体基因名称）在番茄果实成熟和品质形成中的生物学功能。
