Wu Mengbo, Liu Kaidong, Li Honghai, Li Ying, Zhu Yunqi, Su Dan, Zhang Yaoxin, Deng Heng, Wang Yikui, Liu Mingchun
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, China.
Life Science and Technology School, Lingnan Normal University, Zhanjiang, 524048, China.
Hortic Res. 2023 Dec 18;11(2):uhad275. doi: 10.1093/hr/uhad275. eCollection 2024 Feb.
The phytohormone ethylene is well known for its important role in the ripening of climacteric fruit, such as tomato (). However, the role and mode of action of other plant hormones in climacteric fruit ripening regulation are not fully understood. Here, we showed that exogenous GA treatment or increasing endogenous gibberellin content by overexpressing the gibberellin synthesis gene specifically in fruit tissues delayed tomato fruit ripening, whereas treatment with the GA biosynthesis inhibitor paclobutrazol (PAC) accelerated fruit ripening. Moreover, exogenous ethylene treatment cannot completely reverse the delayed fruit ripening phenotype. Furthermore, exogenous GA treatment of ethylene signalling mutant () or -overexpressing lines still delayed fruit ripening, suggesting that GA involved in fruit ripening partially depends on ethylene. Transcriptome profiling showed that gibberellin affect the ripening of fruits by modulating the metabolism and signal transduction of multiple plant hormones, such as auxin and abscisic acid, in addition to ethylene. Overall, the results of this study provide new insight into the regulation of gibberellin in fruit ripening through mediating multiple hormone signals.
植物激素乙烯因其在跃变型果实（如番茄）成熟过程中的重要作用而广为人知。然而，其他植物激素在跃变型果实成熟调控中的作用和作用方式尚未完全了解。在此，我们表明，外源赤霉素处理或通过在果实组织中特异性过表达赤霉素合成基因来增加内源赤霉素含量，可延迟番茄果实成熟，而用赤霉素生物合成抑制剂多效唑（PAC）处理则加速果实成熟。此外，外源乙烯处理不能完全逆转果实成熟延迟的表型。此外，用外源赤霉素处理乙烯信号突变体或过表达系仍会延迟果实成熟，这表明赤霉素参与果实成熟部分依赖于乙烯。转录组分析表明，除乙烯外，赤霉素还通过调节多种植物激素（如生长素和脱落酸）的代谢和信号转导来影响果实成熟。总体而言，本研究结果为赤霉素通过介导多种激素信号调控果实成熟提供了新的见解。
