Batra S K, Miller W L
Endocrinology. 1985 Dec;117(6):2443-8. doi: 10.1210/endo-117-6-2443.
Progesterone (P4) regulation of FSH production was studied in dispersed ovine pituitary cell culture. FSH secretion into culture media was decreased by 30-70% in the presence of 10(-7) M P4. The ED50 of P4 was 2 X 10(-10) M. Decreased FSH secretion was observed as early as 9-12 h after treatment with P4 (10(-7) M). FSH secretion rebounded to control levels after P4 was removed for 4 days. The intracellular FSH content also decreased after P4 treatment, suggesting that decreased FSH secretion is due to decreased biosynthesis. Two progesterone metabolites, 5 alpha-dihydroprogesterone and 17 alpha-hydroxyprogesterone, showed relative activities of 39% and 28%, respectively, compared to P4. Although testosterone (10(-7) M) inhibited FSH secretion by 10%, it may have done so only via conversion to estradiol. Dihydrotestosterone, androstanedione, and cortisol had no effect on FSH secretion. P4 consistently showed activity in 2-day-old ovine culture, but its effect essentially disappeared by day 10 or 12 of culture. When insulin was included in the culture medium (medium 199 plus 10% charcoal-treated anestrous ewe serum), the effect of P4 was maintained for up to 20 days, and FSH production, in general, was augmented with insulin. These results indicate that P4 can specifically inhibit FSH production in ovine pituitary cell culture, but there is no long term maintenance of the P4 response unless insulin is present. Finally, it is known that 17 beta-estradiol also inhibits FSH production in ovine pituitary cultures. Therefore, our results establish that FSH production is a phenomenon that is regulated similarly by both estradiol and P4.
在分散的绵羊垂体细胞培养中研究了孕酮（P4）对促卵泡激素（FSH）产生的调节作用。在存在10⁻⁷ M P4的情况下，FSH分泌到培养基中的量减少了30 - 70%。P4的半数有效剂量（ED50）为2×10⁻¹⁰ M。在用P4（10⁻⁷ M）处理后最早9 - 12小时就观察到FSH分泌减少。在P4去除4天后，FSH分泌反弹至对照水平。P4处理后细胞内FSH含量也降低，这表明FSH分泌减少是由于生物合成减少所致。与P4相比，两种孕酮代谢产物，5α - 二氢孕酮和17α - 羟孕酮的相对活性分别为39%和28%。尽管睾酮（10⁻⁷ M）使FSH分泌减少了10%，但它可能仅通过转化为雌二醇才产生这种作用。双氢睾酮、雄烯二酮和皮质醇对FSH分泌没有影响。P4在2日龄绵羊培养物中始终表现出活性，但其作用在培养第10天或12天时基本消失。当培养基（M199加10%经活性炭处理的乏情母羊血清）中加入胰岛素时，P4的作用可维持长达20天，并且一般来说，FSH的产生会因胰岛素而增加。这些结果表明，P4可特异性抑制绵羊垂体细胞培养中FSH的产生，但除非存在胰岛素，否则P4反应无法长期维持。最后，已知17β - 雌二醇也抑制绵羊垂体培养物中FSH的产生。因此，我们的结果表明，FSH的产生是一种受雌二醇和P4类似调节的现象。
