大豆苷元通过抑制炎性细胞因子和肌肉萎缩相关基因的表达抑制肌肉萎缩。

Daidzein Inhibits Muscle Atrophy by Suppressing Inflammatory Cytokine- and Muscle Atrophy-Related Gene Expression.

机构信息

Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.

出版信息

Nutrients. 2024 Sep 13;16(18):3084. doi: 10.3390/nu16183084.

DOI:10.3390/nu16183084

PMID:39339684

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11434955/

Abstract

BACKGROUND

Sarcopenic obesity, which is associated with a poorer prognosis than that of sarcopenia alone, may be positively affected by soy isoflavones, known inhibitors of muscle atrophy. Herein, we hypothesize that these compounds may prevent sarcopenic obesity by upregulating the gut metabolites with anti-inflammatory effects.

METHODS

To explore the effects of soy isoflavones on sarcopenic obesity and its mechanisms, we employed both in vivo and in vitro experiments. Mice were fed a high-fat, high-sucrose diet with or without soy isoflavone supplementation. Additionally, the mouse C2C12 myotube cells were treated with palmitic acid and daidzein in vitro.

RESULTS

The isoflavone considerably reduced muscle atrophy and the expression of the muscle atrophy genes in the treated group compared to the control group (, = 0.0012; , < 0.0001; , < 0.0001; , = 0.1343). Elevated levels of daidzein were found in the muscles and feces of the experimental group compared to the control group (feces, 0.0122; muscle, 0.0020). The real-time PCR results demonstrated that the daidzein decreased the expression of the palmitate-induced inflammation and muscle atrophy genes in the C2C12 myotube cells (, 0.0201; , 0.0008; , 0.0001; , 0.0002; , 0.0114; , 0.0001). Additionally, it reduced the palmitate-induced protein expression related to the muscle atrophy in the C2C12 myotube cells (, 0.0078; MuRF1, 0.0119).

CONCLUSIONS

The daidzein suppressed inflammatory cytokine- and muscle atrophy-related gene expression in the C2C12 myotubes, thereby inhibiting muscle atrophy.

摘要

背景

与单纯的肌肉减少症相比，与预后较差相关的肌肉减少性肥胖症可能会受到大豆异黄酮的积极影响，大豆异黄酮是肌肉萎缩的已知抑制剂。在此，我们假设这些化合物可能通过上调具有抗炎作用的肠道代谢物来预防肌肉减少性肥胖症。

方法

为了探讨大豆异黄酮对肌肉减少性肥胖症及其机制的影响，我们进行了体内和体外实验。给小鼠喂食高脂肪、高蔗糖饮食，同时补充或不补充大豆异黄酮。此外，体外用棕榈酸和大豆苷元处理 C2C12 肌管细胞。

结果

与对照组相比，异黄酮可显著减少肌肉萎缩和肌肉萎缩基因的表达（，=0.0012；，<0.0001；，<0.0001；，=0.1343）。与对照组相比，实验组肌肉和粪便中的大豆苷元水平升高（粪便，0.0122；肌肉，0.0020）。实时 PCR 结果表明，大豆苷元降低了 C2C12 肌管细胞中棕榈酸诱导的炎症和肌肉萎缩基因的表达（，0.0201；，0.0008；，0.0001；，0.0002；，0.0114；，0.0001）。此外，它还降低了 C2C12 肌管细胞中与肌肉萎缩相关的棕榈酸诱导的蛋白表达（，0.0078；MuRF1，0.0119）。