Tzovara Irene, Papadatou Ioanna, Tzanoudaki Marianna, Piperi Christina, Kanaka-Gantenbein Christina, Spoulou Vana
Immunobiology and Vaccinology Research Laboratory, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece.
First Department of Pediatrics, School of Medicine, "Aghia Sophia" Children's Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Vaccines (Basel). 2024 Sep 7;12(9):1024. doi: 10.3390/vaccines12091024.
Pneumococcal vaccination schedules are traditionally assessed based on the antibody response. The Memory B Cell (MBC) response has been less studied, despite its role in the magnitude and longevity of protection. We compared the immune response to different vaccination schedules with the 13-valent Pneumococcal Conjugate Vaccine (PCV13) and investigated the relationship between MBCs and the antibody response. Total and pneumococcal serotype (PS)-specific MBCs, their subsets and PS-specific IgG antibodies induced by a 3 + 0 (group A), 2 + 1 (group B) or 3 + 1 (group C) schedule in healthy infants were studied before and 1 month after the last PCV13. The relatively immature IgM+IgD+ MBC subset was the predominant subset in all groups but was larger in group A compared to group B and group C, indicating that age might be a significant parameter of the composition of the MBC pool. PS-specific MBCs at baseline were higher in group A, but they increased significantly only in the groups receiving the booster schedules (groups B and C). PS-specific IgM-only MBCs at baseline positively corelated with the antibody response and the PS-specific swIg MBCs post-immunization. Our findings illustrate the importance of a booster dose for the enrichment of PS-specific immunological memory. IgM-only MBCs and swIg MBCs may serve as additional correlates of vaccine-induced protection.
肺炎球菌疫苗接种计划传统上是根据抗体反应来评估的。尽管记忆B细胞（MBC）反应在保护的强度和持久性方面发挥着作用，但对其研究较少。我们比较了13价肺炎球菌结合疫苗（PCV13）不同接种计划的免疫反应，并研究了MBC与抗体反应之间的关系。在健康婴儿最后一剂PCV13接种前和接种后1个月，研究了3 + 0（A组）、2 + 1（B组）或3 + 1（C组）接种计划诱导的总MBC和肺炎球菌血清型（PS）特异性MBC、其亚群以及PS特异性IgG抗体。相对不成熟的IgM+IgD+ MBC亚群在所有组中都是主要亚群，但A组比B组和C组更大，这表明年龄可能是MBC库组成的一个重要参数。A组基线时的PS特异性MBC较高，但仅在接受加强接种计划的组（B组和C组）中显著增加。基线时仅PS特异性IgM的MBC与抗体反应以及免疫后的PS特异性分泌型IgM MBC呈正相关。我们的研究结果说明了加强剂量对于丰富PS特异性免疫记忆的重要性。仅IgM的MBC和分泌型IgM的MBC可能作为疫苗诱导保护的额外相关指标。
