Li Kai, Liu Yongzhen, Liu Changjun, Zhang Yanping, Cui Hongyu, Qi Xiaole, Zhang Jiayong, Xu Jia, Wang Suyan, Chen Yuntong, Duan Yulu, Gao Yulong, Wang Xiaomei
Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610299, China.
Vaccines (Basel). 2024 Sep 13;12(9):1047. doi: 10.3390/vaccines12091047.
The chicken infectious anemia virus (CIAV) has been reported in major poultry-producing countries and poses a significant threat to the poultry industry worldwide. In this study, two Marek's disease virus (MDV) recombinants, rMDV-CIAV-1 and rMDV-CIAV-2, were generated by inserting the CIAV VP1 and VP2 genes into the MDV vaccine strain 814 at the US2 site using the fosmid-based rescue system. For rMDV-CIAV-1, an internal ribosome entry site was inserted between VP1 and VP2, so that both proteins were produced from a single open reading frame. In rMDV-CIAV-2, VP1 and VP2 were cloned into different open reading frames and inserted into the MDV genome. The recombinant viruses simultaneously expressed VP1 and VP2 in infected chicken embryo fibroblasts and exhibited growth kinetics similar to those of the parent MDV. The two recombinant viruses induced antibodies against CIAV in chickens. A single dose of the recombinant viruses provided strong protection against CIAV-induced anemia in chickens. These recombinant VP1- and VP2-expressing MDVs are potential vaccines against CIAV in chickens.
鸡传染性贫血病毒（CIAV）已在主要家禽生产国被报道，对全球家禽业构成重大威胁。在本研究中，通过使用基于fosmid的拯救系统，将CIAV的VP1和VP2基因插入MDV疫苗株814的US2位点，构建了两种马立克氏病病毒（MDV）重组体，即rMDV-CIAV-1和rMDV-CIAV-2。对于rMDV-CIAV-1，在VP1和VP2之间插入了一个内部核糖体进入位点，使得两种蛋白由一个单一的开放阅读框产生。在rMDV-CIAV-2中，VP1和VP2被克隆到不同的开放阅读框中并插入MDV基因组。重组病毒在感染的鸡胚成纤维细胞中同时表达VP1和VP2，并表现出与亲本MDV相似的生长动力学。这两种重组病毒在鸡体内诱导产生了针对CIAV的抗体。单剂量的重组病毒为鸡提供了针对CIAV诱导的贫血的强大保护。这些表达重组VP1和VP2的MDV是鸡抗CIAV的潜在疫苗。
