Analysis of the implication of steroid 5 alpha-reductase 3 on prognosis and immune microenvironment in Liver Hepatocellular Carcinoma.

INTRODUCTION

This study combined the bioinformatics and experiment-related technologies to analyze the impact of steroid 5 alpha-reductase 3 (SRD5A3) on the prognosis and immune microenvironment of Liver Hepatocellular Carcinoma (LIHC).

METHOD

Gene expression and clinical data were obtained from public databases. The prognosis was evaluated using survival, multifactor Cox, enrichment, and mutation analyses. This was then verified through experiments.

RESULTS

The expression level of in LIHC tissues was significantly higher than that in the adjacent tissues. Kaplan-Meier survival analysis showed that high expression was associated with poor overall survival (OS) and short progression-free survival in patients with LIHC. Multivariate Cox regression analysis revealed that positive expression was an independent risk factor for OS in patients with LIHC. Expression of was negatively correlated with immune cell infiltration of CD4+ T, CD8+ T, and B cells. GO and KEGG enrichment analyses showed that was significantly enriched in signaling- and tumor metastasis-related pathways. Nomogram and calibration curve showed that the predicted performance of the model was consistent with the actual results. results confirmed that knockdown inhibited the migration, invasion, and proliferation of LIHC cells.

CONCLUSIONS

is actively expressed in LIHC, and positive expression of is an independent risk factor for different prognoses in patients with LIHC. can promote the proliferation, migration, and invasion of liver cancer cells and is related to short immune infiltration in patients with LIHC.