Schepens Eye Research Institute, Department of Ophthalmology, Massachusetts Eye and Ear, Harvard Medical School, Boston, USA.
Schepens Eye Research Institute, Department of Ophthalmology, Massachusetts Eye and Ear, Harvard Medical School, Boston, USA.
Biochem Biophys Res Commun. 2024 Nov 19;734:150744. doi: 10.1016/j.bbrc.2024.150744. Epub 2024 Sep 25.
To collect tear fluid biomarkers from contact lenses (CLs) and determine the impact of CL wear duration.
Rabbits were fitted with commercial etafilcon A CLs, which were collected after 1 min, 4 and 8 h (n = 4/time point). Tear fluid proteins and cytokines were extracted from the CLs and quantified. An exploratory comparison was performed between CLs and Schirmer Strips (SS) for a 1 min duration.
The concentration of MUC5AC was significantly higher after 4 h of CL wear. The expression of all investigated cytokines (IL-1α, IL-1β, IL-8, IL-17A, IL-21, Leptin, MIP-1β, MMP-9, NCAM-1, and TNF-α) was detectable after 1 min of CL wear, and over time, all showed significant variations throughout the 8-h CL wear period. Notably, IL-1α significantly increased by 8 h of CL wear, while MMP-9 decreased. Albumin and lysozyme did not show significant variations with CL wear. Differences between CLs and SS after 1 min were statistically significant for albumin, Leptin, TNF-α, IL-1α, IL-1β, and IL-8.
The duration of CL wear significantly affects the collection of some tear fluid biomarkers. Albumin, MUC5AC, and cytokines may have individual and synergistic diagnostic or prognostic potential. CLs and SS were similar for lysozyme and MUC5AC but differed in the collection of albumin and some cytokines. CLs are a viable tear fluid collection method for biomarker analyses and can be immediately added as a routine clinical test by being FDA-approved medical devices.
从隐形眼镜（CL）中收集泪液生物标志物，并确定 CL 佩戴时间的影响。
将商业醋酸纤维素（etafilcon A）CL 佩戴在兔子眼睛上，在 1 分钟、4 小时和 8 小时后收集（n=4/时间点）。从 CL 中提取并定量泪液蛋白和细胞因子。对 1 分钟时长的 CL 和 Schirmer 条（SS）进行探索性比较。
CL 佩戴 4 小时后，MUC5AC 的浓度明显升高。所有检测到的细胞因子（IL-1α、IL-1β、IL-8、IL-17A、IL-21、瘦素、MIP-1β、MMP-9、NCAM-1 和 TNF-α）在 CL 佩戴 1 分钟后即可检测到，随着时间的推移，所有这些细胞因子在 8 小时 CL 佩戴期间均呈现显著变化。值得注意的是，CL 佩戴 8 小时后，IL-1α 显著增加，而 MMP-9 减少。白蛋白和溶菌酶的 CL 佩戴变化无统计学意义。CL 佩戴 1 分钟后，CL 与 SS 之间的差异在白蛋白、瘦素、TNF-α、IL-1α、IL-1β 和 IL-8 方面具有统计学意义。
CL 佩戴时间显著影响一些泪液生物标志物的采集。白蛋白、MUC5AC 和细胞因子可能具有个体和协同的诊断或预后潜力。CL 和 SS 对于溶菌酶和 MUC5AC 相似，但在白蛋白和某些细胞因子的采集方面有所不同。CL 是一种可行的泪液采集方法，可作为经 FDA 批准的医疗器械，立即添加为常规临床检测。
