State Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China; Liaoning Key Laboratory for Mass Spectrometry Technology and Instrumentation, Dalian, 116023, PR China.
State Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, PR China; Liaoning Key Laboratory for Mass Spectrometry Technology and Instrumentation, Dalian, 116023, PR China.
Talanta. 2025 Jan 1;282:126965. doi: 10.1016/j.talanta.2024.126965. Epub 2024 Sep 26.
As a common by-product during the production of alcoholic beverages, such as Chinese liquor, ethyl carbamate (EC) poses potential genotoxicity and is associated with the risk of various cancers. Hence, rapidly and accurately measuring the content of EC in liquor is critical to assess the product quality and risks of mass samples during the production process. In this study, a feasible method based on fast gas chromatography photoionization-induced chemical ionization mass spectrometry (FastGC-PICI-TOFMS) was developed for the analysis of EC in Chinese liquor. The rapid separation of EC in Chinese liquor was conducted using FastGC based on a thermostatic column set at 150 °C to eliminate the interferences of matrix effects. The PICI-TOFMS could realize accurate quantification of EC without any sample pre-treatment due to the efficient ionization of EC by the PICI source. As a result, the total analysis time for EC in Chinese liquor was less than 4 min. The limit of detection (LOD) for EC was 4.4 μg L. And the intra-day and inter-day precision were 3.2%-3.7 % and 1.6 %, respectively. Finally, the ability of the proposed method was preliminarily proved by high-throughput and accurate measurement of EC in four different flavors of Chinese liquors.
作为酒精饮料生产过程中的常见副产物，如中国白酒中的氨基甲酸乙酯（EC）具有潜在的遗传毒性，并与各种癌症的风险相关。因此，快速、准确地测量白酒中 EC 的含量对于评估产品质量和生产过程中大量样品的风险至关重要。在本研究中，开发了一种基于快速气相色谱光离子化-诱导化学电离飞行时间质谱法（FastGC-PICI-TOFMS）的可行方法，用于分析中国白酒中的 EC。基于恒温柱（设置在 150°C）的 FastGC 可快速分离中国白酒中的 EC，以消除基质效应的干扰。PICI-TOFMS 可实现 EC 的准确定量，而无需任何样品预处理，因为 PICI 源可有效地对 EC 进行离子化。因此，中国白酒中 EC 的总分析时间不到 4 分钟。EC 的检出限（LOD）为 4.4μg/L。日内和日间精密度分别为 3.2%-3.7%和 1.6%。最后，通过高通量和准确测量四种不同风味的中国白酒中的 EC，初步验证了该方法的能力。
