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在核酸外切酶I和V均缺失的大肠杆菌菌株中大肠菌素E1的自发诱导。

Spontaneous induction of colicin E1 in Escherichia coli strains deficient in both exonucleases I and V.

作者信息

Bassett C L, Kushner S R

出版信息

J Bacteriol. 1985 Dec;164(3):1362-5. doi: 10.1128/jb.164.3.1362-1365.1985.

Abstract

Colicin E1 synthesis is spontaneously induced in pRSF2124-carrying strains of Escherichia coli deficient in exonucleases I (sbcB) and V (recB recC). In contrast, the specific activity of beta-lactamase, which is also encoded by pRSF2124, is not affected by the absence of these enzymes. These results suggest that colicin E1 induction is specific and does not result either from a significant change in overall plasmid transcription or copy number. Furthermore, the level of spontaneous induction was similar to that obtained with mitomycin C.

摘要

在缺乏核酸外切酶I(sbcB)和V(recB recC)的携带pRSF2124的大肠杆菌菌株中,大肠杆菌素E1的合成会自发诱导。相比之下,同样由pRSF2124编码的β-内酰胺酶的比活性不受这些酶缺失的影响。这些结果表明,大肠杆菌素E1的诱导是特异性的,并非由整体质粒转录或拷贝数的显著变化所致。此外,自发诱导水平与用丝裂霉素C获得的水平相似。

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本文引用的文献

2
Production of colicine by single bacteria.单个细菌产生大肠杆菌素。
Nature. 1959 Aug 1;184:337-9. doi: 10.1038/184337a0.
8
Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱,第7版。
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
9
cea-kil operon of the ColE1 plasmid.ColE1质粒的cea-kil操纵子
J Bacteriol. 1983 Mar;153(3):1479-85. doi: 10.1128/jb.153.3.1479-1485.1983.

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