Bassett C L, Kushner S R
J Bacteriol. 1985 Dec;164(3):1362-5. doi: 10.1128/jb.164.3.1362-1365.1985.
Colicin E1 synthesis is spontaneously induced in pRSF2124-carrying strains of Escherichia coli deficient in exonucleases I (sbcB) and V (recB recC). In contrast, the specific activity of beta-lactamase, which is also encoded by pRSF2124, is not affected by the absence of these enzymes. These results suggest that colicin E1 induction is specific and does not result either from a significant change in overall plasmid transcription or copy number. Furthermore, the level of spontaneous induction was similar to that obtained with mitomycin C.
在缺乏核酸外切酶I(sbcB)和V(recB recC)的携带pRSF2124的大肠杆菌菌株中,大肠杆菌素E1的合成会自发诱导。相比之下,同样由pRSF2124编码的β-内酰胺酶的比活性不受这些酶缺失的影响。这些结果表明,大肠杆菌素E1的诱导是特异性的,并非由整体质粒转录或拷贝数的显著变化所致。此外,自发诱导水平与用丝裂霉素C获得的水平相似。