Lebrun Labs LLC, 3301 E. Miraloma Ave., Ste. 194, Anaheim, CA 92806, USA.
Lebrun Labs LLC, 3301 E. Miraloma Ave., Ste. 194, Anaheim, CA 92806, USA.
Toxicol In Vitro. 2024 Dec;101:105944. doi: 10.1016/j.tiv.2024.105944. Epub 2024 Sep 26.
The purpose of this study is to evaluate a novel macromolecular test method for the identification of dermal corrosives. The simple in chemico test procedure involves allowing the material to be tested to interact with a skin biomarker for corrosivity and then adding a detection reagent. The corrosivity of the test substance is predicted based on the measured macromolecular damage, which results in reduced optical density of the detection reagent as compared with controls. This study aims to determine if such an extremely simple, cell-free test method can accurately identify dermal corrosives. To determine predictivity and repeatability, we tested 60 chemicals (30 in vivo dermal corrosives and 30 in vivo dermal noncorrosives; all tested in triplicate) representative of a broad range of chemical classes, functional groups, mixtures, and levels of toxicity. Validation results indicate the GHS multicategory and packing group assignment accuracy is on par with that of the Reconstructed Human Epidermis test method and the Membrane Barrier test method and for the global identification of corrosives, the method has a considerably higher accuracy (98 % vs. ∼80 %).
本研究旨在评估一种新型的大分子测试方法，用于鉴定皮肤腐蚀性物质。该方法在化学方面的测试程序非常简单，只需让待测试的物质与皮肤腐蚀性生物标志物相互作用，然后添加检测试剂。根据所测量的大分子损伤，预测测试物质的腐蚀性，与对照相比，检测试剂的光密度降低。本研究旨在确定如此简单、无细胞的测试方法是否能够准确识别皮肤腐蚀性物质。为了确定预测性和可重复性，我们测试了 60 种化学物质（30 种体内皮肤腐蚀性物质和 30 种体内皮肤非腐蚀性物质；均重复测试 3 次），代表了广泛的化学类别、功能基团、混合物和毒性水平。验证结果表明，GHS 多类别和包装组的分配准确性与重建人体表皮测试方法和膜屏障测试方法相当，并且该方法对腐蚀性物质的全球识别具有更高的准确性（98%比约 80%）。
