Linden M, Oredsson S M, Anehus S, Heby O
J Cell Biochem. 1985;29(2):105-13. doi: 10.1002/jcb.240290206.
The ornithine decarboxylase (ODC) activity of Ehrlich ascites tumor cells was almost completely inhibited by treatment with either putrescine (10 mM) or 1,3-diaminopropane (5 mM). 1,3-Diaminopropane treatment eradicated the cellular content of putrescine and reduced that of spermidine and spermine. Putrescine treatment caused a dramatic increase in cellular putrescine content and a temporary decrease in spermidine and spermine content. Despite the fact that 1,3-diaminopropane and putrescine inhibited the ODC activity more effectively than did alpha-difluoromethylornithine (DFMO), an enzyme-activated irreversible inhibitor of ODC, they were considerably less antiproliferative in action. However, as compared to DFMO the diamines were less effective in reducing the total polyamine (putrescine + spermidine + spermine) content of the cells.
用腐胺（10 mM）或1,3 - 二氨基丙烷（5 mM）处理艾氏腹水瘤细胞后，其鸟氨酸脱羧酶（ODC）活性几乎完全被抑制。1,3 - 二氨基丙烷处理消除了细胞内腐胺的含量，并降低了亚精胺和精胺的含量。腐胺处理导致细胞内腐胺含量急剧增加，亚精胺和精胺含量暂时降低。尽管1,3 - 二氨基丙烷和腐胺比α - 二氟甲基鸟氨酸（DFMO，一种ODC的酶激活不可逆抑制剂）更有效地抑制ODC活性，但它们的抗增殖作用要小得多。然而，与DFMO相比，二胺类在降低细胞总多胺（腐胺+亚精胺+精胺）含量方面效果较差。
