Department of Plant Breeding and Genetics, B. Z. University Multan, Multan, Pakistan.
Institute of Plant Breeding & Biotechnology (IPBB), Muhammad Nawaz Shareef University of Agriculture, Multan, 60000, Pakistan.
BMC Plant Biol. 2024 Sep 28;24(1):886. doi: 10.1186/s12870-024-05610-7.
Climate change has been drastically affecting cotton not only in Pakistan but also all over the world. Normally cotton is known as heat tolerant when compared with other crops, but if the high temperature occurs during flowering period the yield decreases significantly. Marker assisted gene pyramiding provides a sustainable solution to improve heat tolerance. A total of seven genotypes were developed by a series of crossing seven tolerant genotypes over the period of three years. Tolerant genotypes were selected by screening for important transcription factors (GHSP26, HSP3, HSFA2, DREB1A, HSP101, DREB2A, GhNAC2, HSPCB, GhWRKY41, TPS, GbMYB5, ANNAT8, GhMPK17, GhMKK1, GhMKK3, GhMPK2, HSC70, APX1 and GhPP2A1). The seven genotypes were evaluated under normal and heat stress in a multi-year trial. The traits related to heat tolerance, such as cell membrane stability, relative water content, excised leaf water loss, plant height, number of nodes, internodal length, number of buds, number of bolls and leaf area was observed under normal and heat stress conditions. The developed genotypes showed improvement in cell membrane stability and relative water content under heat stress. The genotypes [(VH-305×MNH-886)×MNH-1035)×NIAB-78)], [(MNH-1035×MNH-886)×MNH-886)×SM-431] and [(MNH-1035×MNH-886)×MNH-886)×SS-32] depicted heat tolerance and could be used as heat tolerant material for variety development in breeding programs.
气候变化不仅对巴基斯坦，而且对全世界的棉花都产生了巨大影响。与其他作物相比，棉花通常被认为具有耐热性，但如果高温出现在开花期，产量会显著下降。标记辅助基因聚合提供了一种提高耐热性的可持续解决方案。在三年内通过一系列杂交七种耐病基因型，总共开发了七个基因型。通过筛选重要的转录因子（GHSP26、HSP3、HSFA2、DREB1A、HSP101、DREB2A、GhNAC2、HSPCB、GhWRKY41、TPS、GbMYB5、ANNAT8、GhMPK17、GhMKK1、GhMKK3、GhMPK2、HSC70、APX1 和 GhPP2A1）来选择耐病基因型。在多年试验中，对这七个基因型在正常和热胁迫下进行了评估。在正常和热胁迫条件下观察到与耐热性相关的性状，如细胞膜稳定性、相对水含量、离体叶片水分损失、株高、节数、节间长度、芽数、棉铃数和叶面积。所开发的基因型在热胁迫下表现出细胞膜稳定性和相对水含量的提高。基因型[(VH-305×MNH-886)×MNH-1035)×NIAB-78)]、[(MNH-1035×MNH-886)×MNH-886)×SM-431]和[(MNH-1035×MNH-886)×MNH-886)×SS-32]表现出耐热性，可以作为耐热材料用于品种选育计划。
