多超排卵对卵母细胞和后代转录及基因组甲基化的影响。

Effects of multisuperovulation on the transcription and genomic methylation of oocytes and offspring.

机构信息

Reproductive Medicine Center, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, China.

College of Life Sciences, Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement, in Universities of Shandong, Qingdao Agricultural University, Qingdao, 266109, China.

出版信息

Clin Epigenetics. 2024 Sep 28;16(1):135. doi: 10.1186/s13148-024-01746-3.

DOI:10.1186/s13148-024-01746-3

PMID:39342274

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11439255/

Abstract

BACKGROUND

Controlled ovarian stimulation is a common skill of assisted reproductive technologies (ARTs). In the clinic, some females would undergo more than one controlled ovarian stimulation cycle. However, few studies have focused on the influence of multi-superovulation on oocytes and offspring.

RESULTS

Here, we found that multi-superovulation disrupted the transcriptome of oocytes and that the differentially expressed genes (DEGs) were associated mainly with metabolism and fertilization. The disruption of mRNA degradation via poly (A) size and metabolism might be a reason for the reduced oocyte maturation rate induced by repeated superovulation. Multi-superovulation results in hypo-genomic methylation in oocytes. However, there was an increase in the methylation level of CGIs. The DMRs are not randomly distributed in genome elements. Genes with differentially methylated regions (DMRs) in promoters are enriched in metabolic pathways. With increasing of superovulation cycles, the glucose and insulin tolerance of offspring is also disturbed.

CONCLUSIONS

These results suggest that multi-superovulation has adverse effects on oocyte quality and offspring health.

摘要

背景

控制性卵巢刺激是辅助生殖技术（ART）的常见技能。在临床上，一些女性会经历多次控制性卵巢刺激周期。然而，很少有研究关注多超排卵对卵子和后代的影响。

结果

在这里，我们发现多超排卵会破坏卵子的转录组，差异表达基因（DEGs）主要与代谢和受精有关。通过 poly(A) 大小和代谢破坏 mRNA 降解可能是重复超排卵引起的卵母细胞成熟率降低的原因。多超排卵导致卵母细胞低基因组甲基化。然而，CGIs 的甲基化水平增加。DMR 不是随机分布在基因组元件中的。启动子中具有差异甲基化区域（DMR）的基因富集在代谢途径中。随着超排卵周期的增加，后代的葡萄糖和胰岛素耐量也受到干扰。