Manger B, Weiss A, Weyand C, Goronzy J, Stobo J D
J Immunol. 1985 Dec;135(6):3669-73.
Soluble antibodies against the T3/antigen receptor complex alone are not sufficient to induce proliferation and interleukin 2 expression by T lymphocytes. An additional requirement is the presence of accessory cells (AC). In this model, AC provide at least two functions required for T cell activation: 1) the surface interaction of T3 antibodies with Fc receptors on the AC surface and 2) the production of soluble mediators such as interleukin 1 (IL 1). In the experiments reported here, these stimuli are represented by T3 antibodies immobilized onto Sepharose beads and by recombinant IL 1. In this study we investigated differences in activation requirements in resting and activated T cells. Resting T cells were represented by AC-depleted peripheral blood mononuclear cells (PBMC) or the T cell line Jurkat, which phenotypically resembles a resting T cell. Activated T cells were represented by T cell clones and the T cell line HUT 78, which express the activation molecules Ia and the IL 2 receptor (Tac). In resting cells, activation required the presence of three different signals: perturbation of the T3/antigen receptor complex by T3 antibodies, surface redistribution of T3/antigen receptor complexes, and presence of IL 1. In contrast, activated T cells require only perturbation and redistribution of T3/antigen receptor complexes and not IL 1 for the induction of proliferation or IL 2 production. Possible mechanisms of intracellular signaling for these stimuli are discussed.
单独针对T3/抗原受体复合物的可溶性抗体不足以诱导T淋巴细胞增殖和白细胞介素2表达。另外一个必要条件是存在辅助细胞（AC）。在这个模型中，AC提供T细胞激活所需的至少两种功能：1）T3抗体与AC表面的Fc受体的表面相互作用，以及2）可溶性介质如白细胞介素1（IL 1）的产生。在本文报道的实验中，这些刺激分别由固定在琼脂糖珠上的T3抗体和重组IL 1来代表。在本研究中，我们调查了静息T细胞和活化T细胞在激活需求上的差异。静息T细胞由去除AC的外周血单核细胞（PBMC）或T细胞系Jurkat代表，后者在表型上类似于静息T细胞。活化T细胞由T细胞克隆和T细胞系HUT 78代表，它们表达激活分子Ia和白细胞介素2受体（Tac）。在静息细胞中，激活需要三种不同信号的存在：T3抗体对T3/抗原受体复合物的扰动、T3/抗原受体复合物的表面重新分布以及IL 1的存在。相比之下，活化T细胞诱导增殖或产生IL 2仅需要T3/抗原受体复合物的扰动和重新分布，而不需要IL 1。本文还讨论了这些刺激的细胞内信号传导的可能机制。
