University of Jendouba, Higher Institute of Biotechnology of Beja, LR: Functional Physiology and Valorization of Bio-Resources, Beja, Tunisia.
Neurogastroenterol Motil. 2024 Dec;36(12):e14933. doi: 10.1111/nmo.14933. Epub 2024 Sep 30.
The prompt development of obesity/constipation is the most serious problem for both children and adults. Limited studies suggested an association between them but lacked preclinical studies. This study allows to evaluate their crosslink and to compare the aqueous extracts laxative actions of two edible wild fruits of Arbutus unedo (AUAE) and Crataegus monogyna (CMAE) in constipated high-fat diet (HFD) rats.
Wistar rats were divided into experimental groups for 13 weeks: standard (SD) and HFD groups. SD-rats were randomly redivided into 2 groups: SD and SD + Loperamide (LOP, 3 mg/kg, b.w.). HFD-rats were randomly reseparated into HFD-group, (HFD + LOP)-group, [HFD + Yohimbine (YOH, 2 mg/kg, b.w.)]-group, [HFD+ LOP]-groups+ various doses of AUAE or CMAE (75, 150, and 300 mg/kg, b.w.). Diversified indicators were investigated to achieve the expected objectives, including; fecal parameters, gastrointestinal transit (GIT), gastric emptying (GE), oxidative stress-(OxS), blood biochemical analysis, and accompanied small/large bowel histological modification.
The liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS) analysis of AUAE and CMAE allowed the identification of 11 and 6 phenolic compounds, respectively. In HFD-rats, the subsequent dysregulation of GI motility was markedly aggravated. More importantly, with the same way (CMAE and AUAE)-treated groups showed alleviated outcomes for the following: most stool parameters, GIT, and GE were remarkably recovered; a similar recovery pattern was observed in the histopathological structure, OxS, and blood biochemical indicators.
CONCLUSIONS & INFERENCES: Our results experimentally confirmed the crosslink between overweight and constipation and both fruits have potential as functional foods to reduce metabolic risk of the obesity associated with bowel dysmotility.
肥胖/便秘的迅速发展是儿童和成人面临的最严重问题。有限的研究表明两者之间存在关联，但缺乏临床前研究。本研究旨在评估它们之间的关联，并比较两种食用野生水果欧洲越桔（AUAE）和山楂（CMAE）的水提物在便秘高脂饮食（HFD）大鼠中的通便作用。
Wistar 大鼠分为实验组，共 13 周：标准（SD）和 HFD 组。SD 大鼠随机分为 2 组：SD 和 SD+洛哌丁胺（LOP，3mg/kg，bw）。HFD 大鼠随机分为 HFD 组、（HFD+LOP）-组、[HFD+育亨宾（YOH，2mg/kg，bw）]-组、[HFD+LOP]-组+不同剂量的 AUAE 或 CMAE（75、150 和 300mg/kg，bw）。进行了多种指标的研究，以达到预期的目标，包括：粪便参数、胃肠道转运（GIT）、胃排空（GE）、氧化应激（OxS）、血液生化分析以及伴随的小肠/大肠组织学改变。
AUAE 和 CMAE 的液相色谱-电喷雾串联质谱（LC-ESI-MS）分析分别鉴定出 11 和 6 种酚类化合物。在 HFD 大鼠中，随后的 GI 运动失调明显加重。更重要的是，以相同的方式（CMAE 和 AUAE）处理的各组，以下参数明显改善：大多数粪便参数、GIT 和 GE 明显恢复；组织病理学结构、OxS 和血液生化指标也观察到类似的恢复模式。
我们的结果从实验上证实了超重和便秘之间的关联，这两种水果都有可能作为功能性食品，减少与肠道运动障碍相关的肥胖代谢风险。
