进一步的证据表明，肽组氨酸异亮氨酸（PHI）可能在大鼠中作为催乳素释放因子发挥作用。

Further evidence that peptide histidine isoleucine (PHI) may function as a prolactin releasing factor in rats.

作者信息

Ohta H, Kato Y, Tojo K, Shimatsu A, Inoue T, Kabayama Y, Imura H

出版信息

Peptides. 1985 Jul-Aug;6(4):709-12. doi: 10.1016/0196-9781(85)90176-7.

Abstract

Intraventricular administration of peptide histidine isoleucine (PHI) (200 ng, 1, 5 and 10 micrograms/rat) resulted in a significant and dose-related increase in plasma prolactin (PRL) levels in urethane-anesthetized rats and in conscious rats with intraatrial and intraventricular catheters. Intravenous injection of PHI (10 micrograms/rat) also raised plasma PRL levels in these animals. In in vitro studies, PRL release from superfused rat anterior pituitary cells was stimulated by PHI (10(-9), 10(-8) and 10(-7) M) in a dose-related manner. The stimulating effect of PHI (10(-7)M) on PRL release in vitro was as potent as that of vasoactive intestinal polypeptide (VIP) (10(-7) M) and was observed even in the presence of dopamine (10(-7) M). These results suggest that PHI plays a stimulating role in regulating PRL secretion by acting, at least in part, directly on the pituitary in the rat.

摘要

在氨基甲酸乙酯麻醉的大鼠以及带有心房和脑室内导管的清醒大鼠中，脑室内注射肽组氨酸异亮氨酸（PHI）（200纳克、1、5和10微克/大鼠）导致血浆催乳素（PRL）水平显著且呈剂量相关地升高。静脉注射PHI（10微克/大鼠）也使这些动物的血浆PRL水平升高。在体外研究中，PHI（10⁻⁹、10⁻⁸和10⁻⁷摩尔/升）以剂量相关的方式刺激了经灌流的大鼠垂体前叶细胞释放PRL。PHI（10⁻⁷摩尔/升）对体外PRL释放的刺激作用与血管活性肠肽（VIP）（10⁻⁷摩尔/升）相当，并且即使在存在多巴胺（10⁻⁷摩尔/升）的情况下也能观察到。这些结果表明，PHI至少部分地通过直接作用于大鼠垂体，在调节PRL分泌中发挥刺激作用。