Kumari Deepa, Nair Neena, Bedwal R S
Cell and Molecular Biology Laboratory, Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan 302004 India.
Department of Zoology, SS Jain Subodh PG College, Jaipur, India.
Indian J Clin Biochem. 2024 Oct;39(4):539-547. doi: 10.1007/s12291-023-01167-8. Epub 2023 Dec 28.
The study was designed to investigate the effects of dietary zinc deficiency and supplementation on antioxidant system viz. superoxide-dismutase, glutathione reductase, glutathione peroxidase, glutathione- S-transferase, catalase and sulfhydryls levels (GSH, TSH, NPSH and PBSH) in testes of Wistar rats. Pre-pubertal rats were divided into two groups with 6 sub-groups each viz. zinc control (ZC), pair fed (PF), zinc deficient (ZD), zinc control supplementation (ZCS), pair-fed supplementation (PFS) and zinc deficient supplementation (ZDS). Experiments were set for 2- and 4-weeks followed by 4 weeks of zinc supplementation. The zinc deficient group animals exhibited significant decrease in gonado-somatic index (2- and 4- weeks), sulfhydryls levels, GSH, GPx, GR (2 and 4-weeks) and GST concentration (2-weeks). However, after zinc supplementation significant improvement in gonadosomatic index, SH, GSH, antioxidant enzyme levels (GR, GPx, and GST) in deficient groups has been observed. Zinc deficiency during pre-pubertal period affected growth and caused dysregulation of the glutathione antioxidant system. The significant alterations in the levels of antioxidant enzymes and non-enzymatic antioxidant system (GSH and SH) in zinc deficient groups could be due to alleviated generation of free radicals, causative factor for increased oxidative stress which may lead to infertility as oxidative stress is a common pathology seen during infertility. Altered antioxidant system and sulfhydryls levels in testes due to dietary zinc deficiency reflect the significance of optimum zinc for maintaining homeostatic balance in gonadal physiology. Supplementing zinc for 4 weeks could reduce the redox imbalance which may help in alleviating oxidative stress induced alterations in testes.
本研究旨在调查膳食锌缺乏和补充锌对Wistar大鼠睾丸抗氧化系统的影响，即超氧化物歧化酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶、过氧化氢酶和巯基水平（GSH、TSH、NPSH和PBSH）。青春期前大鼠分为两组，每组6个亚组，即锌对照组（ZC）、配对喂养组（PF）、缺锌组（ZD）、锌对照补充组（ZCS）、配对喂养补充组（PFS）和缺锌补充组（ZDS）。实验设定为2周和4周，随后进行4周的锌补充。缺锌组动物在2周和4周时性腺体指数、巯基水平、GSH、GPx、GR（2周和4周）和GST浓度（2周）显著降低。然而，补充锌后，缺锌组的性腺体指数、SH、GSH、抗氧化酶水平（GR、GPx和GST）有显著改善。青春期前锌缺乏影响生长并导致谷胱甘肽抗氧化系统失调。缺锌组抗氧化酶和非酶抗氧化系统（GSH和SH）水平的显著变化可能是由于自由基生成减少，自由基是氧化应激增加的致病因素，氧化应激是不育症常见的病理现象，可能导致不育。膳食锌缺乏导致睾丸抗氧化系统和巯基水平改变，反映了最佳锌对维持性腺生理内稳态平衡的重要性。补充锌4周可减少氧化还原失衡，这可能有助于减轻睾丸中氧化应激诱导的改变。
