Department of Respiratory Medicine and Allergology Kochi Medical School Kochi University, Kochi, Japan.
J Immunol Res. 2024 Sep 19;2024:2020514. doi: 10.1155/2024/2020514. eCollection 2024.
Using flow cytometry, we identified and quantified Group 2 innate lymphocytes, T helper 2 cells, follicular helper T cells, and T helper 17 cells in peripheral blood samples from 49 individuals with asthma. We then conducted cross-sectional analyses to assess relationships between levels of these immune cells and lung function parameters, including the percentage predicted forced expiratory volume in 1 s (%FEV1). We also examined correlations between the proportions of immune cells and type 2 biomarkers.
Proportions of CXCR5 follicular helper T cells in human peripheral blood, as opposed to Group 2 innate lymphoid cells (ILC2) or T helper 2 cells, were significantly higher in cases with %FEV1 < 80% compared to those with %FEV1 ≥ 80%. Further, these proportions correlated negatively with %FEV1 and positively with blood eosinophil counts.
The proportion of circulating follicular helper T cells, but not T helper 2 cells or Group 2 innate lymphoid cells, may reflect the presence of airway obstruction caused by persistent type 2 inflammation.
我们使用流式细胞术鉴定和量化了 49 名哮喘患者外周血样本中的 2 型固有淋巴细胞、辅助性 T 细胞 2 型、滤泡辅助性 T 细胞和辅助性 T 细胞 17 型。然后，我们进行了横断面分析，以评估这些免疫细胞的水平与肺功能参数（包括 1 秒用力呼气量占预计值的百分比[FEV1%]）之间的关系。我们还检查了免疫细胞比例与 2 型生物标志物之间的相关性。
与 2 型固有淋巴细胞（ILC2）或辅助性 T 细胞 2 型相比，外周血中 CXCR5 滤泡辅助性 T 细胞的比例在 FEV1%<80%的病例中明显高于 FEV1%≥80%的病例。此外，这些比例与 FEV1 呈负相关，与血嗜酸性粒细胞计数呈正相关。
循环滤泡辅助性 T 细胞的比例（而非辅助性 T 细胞 2 型或 2 型固有淋巴细胞）可能反映了持续 2 型炎症引起的气道阻塞的存在。
