Ishimori Ayako, Harada Norihiro, Chiba Asako, Harada Sonoko, Matsuno Kei, Makino Fumihiko, Ito Jun, Ohta Shoichiro, Ono Junya, Atsuta Ryo, Izuhara Kenji, Takahashi Kazuhisa, Miyake Sachiko
Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine, Tokyo, Japan.
Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine, Tokyo, Japan; Research Institute for Diseases of Old Ages, Juntendo University Faculty of Medicine and Graduate School of Medicine, Tokyo, Japan; Atopy (Allergy) Research Center, Juntendo University Faculty of Medicine and Graduate School of Medicine, Tokyo, Japan.
Allergol Int. 2017 Apr;66(2):302-309. doi: 10.1016/j.alit.2016.07.005. Epub 2016 Aug 27.
A variety of innate subsets of lymphoid cells such as natural killer (NK) cells, several populations of innate lymphoid cells (ILCs), and mucosal-associated invariant T (MAIT) cells as innate-like T lymphocytes are involved in asthma and may have important effector functions in asthmatic immune responses. In the present study, we investigated whether NK cells, ILCs, and MAIT cells in the peripheral blood of patients with asthma would be associated with clinical asthma parameters.
We recruited 75 adult patients with mild to severe asthma. The peripheral blood mononuclear cells in peripheral venous blood samples from the patients were purified and stained with different combinations of appropriate antibodies. The cells were analyzed by flow cytometry.
The percentage of activated (i.e., CD69) NK cells in the total NK cell population was negatively correlated with FEV% which is calculated by the forced expiratory volume in 1 s (FEV)/the forced vital capacity (FVC). The percentages of CD69 ILC1s and ILC2s were negatively correlated with FEV% and %FEV. The percentage of CD69 ILC3s was positively correlated with BMI, and the percentage of CD69 MAIT cells was negatively correlated with FEV%. Moreover, the percentage of CD69 NK cells, ILC1s, ILC2s, ILC3s, and MAIT cells were positively correlated with each other.
For the first time, our data showed that activated NK cells, ILC1s, ILC2s, ILC3s, and MAIT cells were positively correlated with each other and may be associated with airflow limitation in patients with asthma.
多种先天性淋巴细胞亚群，如自然杀伤（NK）细胞、多个先天性淋巴细胞（ILC）群体以及作为类先天性T淋巴细胞的黏膜相关恒定T（MAIT）细胞，均参与哮喘发病，且可能在哮喘免疫反应中发挥重要效应功能。在本研究中，我们调查了哮喘患者外周血中的NK细胞、ILC和MAIT细胞是否与临床哮喘参数相关。
我们招募了75例轻至重度成年哮喘患者。对患者外周静脉血样本中的外周血单个核细胞进行纯化，并用适当抗体的不同组合进行染色。通过流式细胞术对细胞进行分析。
总NK细胞群体中活化（即CD69）NK细胞的百分比与第1秒用力呼气容积（FEV）/用力肺活量（FVC）计算得出的FEV%呈负相关。CD69 ILC1和ILC2的百分比与FEV%和%FEV呈负相关。CD69 ILC3的百分比与体重指数（BMI）呈正相关，CD69 MAIT细胞的百分比与FEV%呈负相关。此外，CD69 NK细胞、ILC1、ILC2、ILC3和MAIT细胞的百分比彼此呈正相关。
我们的数据首次表明，活化的NK细胞、ILC1、ILC2、ILC3和MAIT细胞彼此呈正相关，且可能与哮喘患者的气流受限有关。
