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使用散斑调制的高速反射共聚焦显微镜。

High-speed reflectance confocal microscopy using speckle modulation.

作者信息

Sugimura Momoka, Marcelino Kenneth, Romero Rafael, Zhao Jingwei, Kim Yongjun, Nessaee Ameer, Kim Kyungjo, Stratton Delaney, Curiel-Lewandrowski Clara, Garfinkel Jason, Rubinstein Gennady, Kang Dongkyun

机构信息

James C. Wyant College of Optical Sciences, University of Arizona, Tucson, Arizona 85721, USA.

Department of Biomedical Engineering, University of Arizona, Tucson, Arizona 85721, USA.

出版信息

Biomed Opt Express. 2024 Jul 29;15(8):4877-4890. doi: 10.1364/BOE.531577. eCollection 2024 Aug 1.

DOI:10.1364/BOE.531577
PMID:39347009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11427182/
Abstract

We developed a spectrally-encoded, line reflectance confocal microscope (RCM) that uses a rotating diffuser to rapidly modulate the illumination speckle pattern. The speckle modulation approach reduced speckle noise while imaging with a spatially coherent light source needed for high imaging speed and cellular resolution. The speckle-modulation RCM device achieved lateral and axial resolutions of 1.1 µm and 2.8 µm, respectively. With an imaging speed of 107 frames/sec, three-dimensional RCM imaging over 300-µm depth was completed within less than 1 second. RCM images of human fingers, forearms, and oral mucosa clearly visualized the characteristic cellular features without any noticeable speckle noise.

摘要

我们开发了一种光谱编码的线反射共聚焦显微镜（RCM），它使用旋转扩散器快速调制照明散斑图案。散斑调制方法在使用高成像速度和细胞分辨率所需的空间相干光源成像时降低了散斑噪声。散斑调制RCM设备的横向和轴向分辨率分别达到了1.1 µm和2.8 µm。成像速度为107帧/秒，在不到1秒的时间内完成了超过300 µm深度的三维RCM成像。人手指、前臂和口腔黏膜的RCM图像清晰地显示了特征性的细胞特征，且没有任何明显的散斑噪声。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/ea44e38771bf/boe-15-8-4877-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/11dc009e5f16/boe-15-8-4877-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/a469a23ae92d/boe-15-8-4877-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/098bc8d52d84/boe-15-8-4877-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/9947fb578ee7/boe-15-8-4877-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/49bb378cf174/boe-15-8-4877-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/eeaf5c8014cf/boe-15-8-4877-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/e2a732d3a076/boe-15-8-4877-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/1d44d053ce34/boe-15-8-4877-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/a8795d544145/boe-15-8-4877-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/ad36215fd844/boe-15-8-4877-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/aba5e9113b10/boe-15-8-4877-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/ea44e38771bf/boe-15-8-4877-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/11dc009e5f16/boe-15-8-4877-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/a469a23ae92d/boe-15-8-4877-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/098bc8d52d84/boe-15-8-4877-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/9947fb578ee7/boe-15-8-4877-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/49bb378cf174/boe-15-8-4877-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/eeaf5c8014cf/boe-15-8-4877-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/e2a732d3a076/boe-15-8-4877-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/1d44d053ce34/boe-15-8-4877-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/a8795d544145/boe-15-8-4877-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/ad36215fd844/boe-15-8-4877-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/aba5e9113b10/boe-15-8-4877-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8592/11427182/ea44e38771bf/boe-15-8-4877-g012.jpg

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