低成本、高速近红外反射共聚焦显微镜。
Low-cost, high-speed near infrared reflectance confocal microscope.
作者信息
Gong Cheng, Kulkarni Nachiket, Zhu Wenbin, Nguyen Christopher David, Curiel-Lewandrowski Clara, Kang Dongkyun
机构信息
College of Optical Sciences, University of Arizona, 1630 E. University Blvd., Tucson, AZ 85721, USA.
University of Arizona Cancer Center, 3838 N. Campbell Ave., Tucson, AZ 85719, USA.
出版信息
Biomed Opt Express. 2019 Jun 21;10(7):3497-3505. doi: 10.1364/BOE.10.003497. eCollection 2019 Jul 1.
We have developed a low-cost, near-infrared (NIR) reflectance confocal microscope (RCM) to overcome challenges in the imaging depth and speed found in our previously-reported smartphone confocal microscope. In the new NIR RCM device, we have used 840 nm superluminescent LED (sLED) to increase the tissue imaging depth and speed. A new confocal detection optics has been developed to maintain high lateral resolution even when a relatively large slit width was used. The material cost of the NIR RCM device was still low, ~$5,200. The lateral resolution was 1.1 µm and 1.3 µm along the vertical and horizontal directions, respectively. Axial resolution was measured as 11.2 µm. confocal images of human forearm skin obtained at the imaging speed of 203 frames/sec clearly visualized characteristic epidermal and dermal cellular features of the human skin.
我们开发了一种低成本的近红外（NIR）反射共聚焦显微镜（RCM），以克服我们之前报道的智能手机共聚焦显微镜在成像深度和速度方面存在的挑战。在新的近红外RCM设备中，我们使用了840 nm的超发光二极管（sLED）来增加组织成像深度和速度。我们开发了一种新的共聚焦检测光学系统，即使使用相对较大的狭缝宽度，也能保持高横向分辨率。近红外RCM设备的材料成本仍然很低，约为5200美元。横向分辨率在垂直和水平方向分别为1.1 µm和1.3 µm。轴向分辨率测量为11.2 µm。以203帧/秒的成像速度获得的人体前臂皮肤共聚焦图像清晰地显示了人体皮肤特征性的表皮和真皮细胞特征。
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