He Qian-Kun, Wang Xue-Yong, Hu Wei, Cai Jing, Chen Peng, Liu Ming-Wei, Wu Yuan-Hua
Department of Neurology, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550001, China.
Department of Neurology, Traditional Chinese Medicine Hospital of Yuxi City, Yuxi, Yunnan, 6527000, China.
Heliyon. 2024 Sep 18;10(18):e38072. doi: 10.1016/j.heliyon.2024.e38072. eCollection 2024 Sep 30.
This study aimed to investigate the effects of Miao medicinal RS3-resistant starch on behavioral performance and substantia nigra neuron apoptosis-related indicators in a rat model of Parkinson's disease (PD). Among the experimental groups, except for the control group, we induced PD rat models by subcutaneous injection of rotenone in the neck and back. After model induction, a 28-day drug intervention was conducted. Various techniques have been employed, including behavioral analysis, Real-time Polymerase Chain Reaction (RT-PCR), western blotting, enzyme-linked immunosorbent assay (ELISA), immunofluorescence, and terminal deoxynucleotidyltransferase-mediated UTP nick-ends. labeling (TUNEL) and Nissl staining to investigate the effect of RS3-resistant starch on the substantia nigra and neuronal apoptosis-related markers in the brains of PD model rats. Our study revealed that RS3, a resistant starch, significantly reduced the climbing time of PD model rats, prolonged their hanging time, lowered the expression levels of the inflammatory factors IL-1β, IL-6, and TNF-α, increased the number of TH-positive neurons in the substantia nigra, and decreased the levels of IL-1β, IL-6, and TNF-α. Furthermore, RS3 elevated the protein expression levels of tyrosine hydroxylase (TH) and Bcl-2 while reducing those of Bax, TLR4, NLRP3,and p-P65, and mitigated apoptosis and morphological changes in dopaminergic neurons in the substantia nigra region. Our results suggest that RS3-resistant starch may offer therapeutic benefits for PD patients with PD by potentially influencing inflammation and apoptosis in the dopaminergic system.
本研究旨在探讨苗药RS3抗性淀粉对帕金森病（PD）大鼠模型行为表现及黑质神经元凋亡相关指标的影响。在实验组中，除对照组外，我们通过在颈部和背部皮下注射鱼藤酮诱导PD大鼠模型。模型诱导后，进行了为期28天的药物干预。采用了多种技术，包括行为分析、实时聚合酶链反应（RT-PCR）、蛋白质印迹法、酶联免疫吸附测定（ELISA）、免疫荧光以及末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）和尼氏染色，以研究RS3抗性淀粉对PD模型大鼠大脑黑质及神经元凋亡相关标志物的影响。我们的研究表明，抗性淀粉RS3显著缩短了PD模型大鼠的攀爬时间，延长了其悬挂时间，降低了炎症因子IL-1β、IL-6和TNF-α的表达水平，增加了黑质中TH阳性神经元的数量，并降低了IL-1β、IL-6和TNF-α的水平。此外，RS3提高了酪氨酸羟化酶（TH）和Bcl-2的蛋白表达水平，同时降低了Bax、TLR4、NLRP3和p-P65的表达水平，并减轻了黑质区域多巴胺能神经元的凋亡和形态变化。我们的结果表明，RS3抗性淀粉可能通过潜在影响多巴胺能系统中的炎症和凋亡，为PD患者提供治疗益处。
