Departments of Pediatric Surgery, Gazi University Faculty of Medicine, Yenimahalle, Ankara, Turkey.
Pathology, Gazi University Faculty of Medicine, Yenimahalle, Ankara, Turkey.
Drug Des Devel Ther. 2024 Sep 25;18:4319-4326. doi: 10.2147/DDDT.S480774. eCollection 2024.
Ischemia-reperfusion (I/R) injury to the testis can lead to organ damage, infertility, and subfertility. The goal of this study was to investigate the effects of fasudil on this devastating condition.
Thirty male Long-Evans rats were divided into five groups: a control group (no torsion), rats administered fasudil (30 mg/kg, no torsion), rats subject to ischemia with no treatment (I) (I/R injury), injured rats that received treatment 1 (T1) (I/R with 30 mg/kg fasudil before detorsion), and injured rats that received treatment 2 (T2) (I/R with 30 mg/kg fasudil after detorsion). Serum levels of TNF-ɑ and IL-6, along with tissue levels of glutathione (GSH), malondialdehyde (MDA), caspase-3, and Johnsen Tubular Biopsy Score (JTBS), were measured.
Group I exhibited significantly higher levels of MDA and caspase-3 than all other groups except T2 (p ˂ 0.05). Although the difference was not statistically significant, Group T2 exhibited lower MDA and caspase-3 levels than Group I (p ˃ 0.05). Additionally, Group I displayed significantly higher TNF-ɑ and IL-6 levels, and lower GSH and JTBS values, than the other groups (p ˂ 0.05).
Our findings indicate that fasudil protects the testis from I/R injury, particularly when administered early.
睾丸的缺血再灌注（I/R）损伤可导致器官损伤、不育和不孕。本研究的目的是探讨法舒地尔对这种破坏性疾病的影响。
30 只雄性 Long-Evans 大鼠分为五组：对照组（无扭转）、法舒地尔（30mg/kg，无扭转）组、无治疗的缺血组（I）（I/R 损伤）、接受 T1 治疗的损伤组（I/R 前用 30mg/kg 法舒地尔治疗）和接受 T2 治疗的损伤组（I/R 后用 30mg/kg 法舒地尔治疗）。测量血清 TNF-ɑ 和 IL-6 水平以及组织谷胱甘肽（GSH）、丙二醛（MDA）、caspase-3 和约翰森管状活检评分（JTBS）水平。
与其他组相比，I 组 MDA 和 caspase-3 水平显著升高，但除 T2 组外（p ˂ 0.05）。虽然差异无统计学意义，但 T2 组的 MDA 和 caspase-3 水平低于 I 组（p ˃ 0.05）。此外，I 组 TNF-ɑ 和 IL-6 水平显著升高，GSH 和 JTBS 值显著降低，与其他组相比差异有统计学意义（p ˂ 0.05）。
我们的研究结果表明，法舒地尔可保护睾丸免受 I/R 损伤，尤其是早期应用时效果更好。
