Liu Yuting, Dang Chi, Yin Dawei, Zheng Ruilin, Zhang Zixu, Zhou Yi, Chen Jiabao
Key Laboratory of Chemical Additives for China National Light Industry, College of Chemistry and Chemical Engineering, Shaanxi University of Science＆Technology, Xìan, 710021, China.
Institute of Frontier Science and Technology Transfer, Shaanxi University of Science＆Technology, Xìan, 710021, China.
J Fluoresc. 2024 Sep 30. doi: 10.1007/s10895-024-03967-4.
In this paper, 1-phenyl-3-ferrocenylenone aminourea Schiff bases were synthesized by a novel method. A multifunctional molecular probe (Probe A) of 1-phenyl-3-ferrocenylenone, carbon-based solid acid, aminourea, and anhydrous ethanol was synthesized by adding them to a vessel at elevated temperatures and refluxing for the synthesis of a multifunctional molecular probe (Probe A) of 1-phenyl-3-ferrocenylenone aminourea Schiff base, and it was found that it recognizes tryptophan (Trp) in solution, and that the catalyst can be reused more than five times after recycling. This method is characterised by low cost, high efficiency, green environment and no waste acid. Fluorescence and UV spectra show that probe A specifically recognizes tryptophan (Trp) without interference by other amino acids or pH and time does not affect it within 45 min. The lowest limit of detection for Trp was 1.307 × 10 mol/L for probe A. The binding ratios of probe A to Trp were measured to be 1:1 by Job's plotting method, respectively. The complexation constant of probe A with Trp was found to be 2.733 × 10 L/mol according to the Benesi-Hildebrand equation. The bonding mechanism was explored through IR spectroscopy and ¹H NMR titration.
本文采用一种新方法合成了1-苯基-3-二茂铁基烯酮氨基脲席夫碱。将1-苯基-3-二茂铁基烯酮、碳基固体酸、氨基脲和无水乙醇加入容器中，在高温下回流，合成了一种多功能分子探针（探针A），即1-苯基-3-二茂铁基烯酮氨基脲席夫碱多功能分子探针，发现它能识别溶液中的色氨酸（Trp），且催化剂回收后可重复使用5次以上。该方法具有成本低、效率高、环境友好且无废酸的特点。荧光和紫外光谱表明，探针A能特异性识别色氨酸（Trp），不受其他氨基酸干扰，在45分钟内pH和时间对其均无影响。探针A对Trp的最低检测限为1.307×10 mol/L。通过Job作图法测得探针A与Trp的结合比分别为1:1。根据Benesi-Hildebrand方程，探针A与Trp的络合常数为2.733×10 L/mol。通过红外光谱和¹H NMR滴定对其结合机理进行了探索。
