诱导小鼠胚胎成纤维细胞（MEFs）衰老。
Inducing Cellular Senescence in Mouse Embryonic Fibroblasts (MEFs).
机构信息
Cellular Plasticity in Age-related Pathologies, Department of Developmental and Stem Cell Biology, Institut Pasteur, Paris, France.
CNRS UMR 3738, Université Paris Cité, Paris, France.
出版信息
Methods Mol Biol. 2025;2857:181-190. doi: 10.1007/978-1-0716-4128-6_17.
PMID:39348066
Abstract
Inducing cellular senescence in mouse embryonic fibroblasts (MEFs) is a robust tool to study the molecular mechanisms underlying senescence establishment and their heterogeneity. This protocol provides a detailed guide to generate MEFs and routinely induce senescence in MEFs using several DNA damage-dependent and DNA damage-independent induction methods.
摘要
诱导小鼠胚胎成纤维细胞（MEFs）衰老为研究衰老建立及其异质性的分子机制提供了一种强有力的工具。本方案提供了一个详细的指南，用于生成 MEFs 并使用几种 DNA 损伤依赖性和非依赖性诱导方法常规诱导 MEFs 衰老。
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