Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Olomouc, Czech Republic.
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Olomouc, Czech Republic
Anticancer Res. 2024 Oct;44(10):4339-4346. doi: 10.21873/anticanres.17263.
BACKGROUND/AIM: Colorectal cancer (CRC) is one of the most widespread malignancies. One of the alternative therapeutic methods appears to be photodynamic therapy (PDT).
This study investigated the efficiency of 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin zinc (ZnTPPS) and chloro-aluminum phthalocyanine disulfonate (ClAlPcS) with two commercial photosensitive compounds 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin (TMPyP) and tetramethylthionine chloride (methylene blue, MB) in PDT for CRC in vitro. In addition to the study of the photodynamic effect on the viability of the colorectal carcinoma cell line HT29, cellular uptake, ROS production, and DNA damage were investigated.
All photosensitizers showed good accumulation within HT29 cells, high efficiency in killing the cells, and a concentration-dependent increase in the production of ROS.
PDT using ZnTPPS and ClAlPcS may be effective in the treatment of CRC, achieving a similar photocytotoxic effect at much lower concentrations compared to MB.
背景/目的：结直肠癌（CRC）是最广泛的恶性肿瘤之一。一种替代的治疗方法似乎是光动力疗法（PDT）。
本研究探讨了 5,10,15,20-四（4-磺基苯基）卟啉锌（ZnTPPS）和氯铝酞菁二磺酸钠（ClAlPcS）与两种商业光敏化合物 5,10,15,20-四（1-甲基吡啶-4-基）卟啉（TMPyP）和氯化四甲基噻嗪（亚甲蓝，MB）在 CRC 体外 PDT 中的效率。除了研究光动力效应对结直肠癌细胞系 HT29 活力的影响外，还研究了细胞摄取、ROS 产生和 DNA 损伤。
所有的光敏剂都在 HT29 细胞内有很好的积累，对细胞有很高的杀伤效率，并且 ROS 的产生呈浓度依赖性增加。
使用 ZnTPPS 和 ClAlPcS 的 PDT 可能对 CRC 的治疗有效，与 MB 相比，在低得多的浓度下就能达到相似的光细胞毒性作用。
