Cracow University of Technology, Faculty of Chemical Engineering and Technology, Warszawska 24, Cracow 31-155, Poland.
Cracow University of Technology, Faculty of Chemical Engineering and Technology, Warszawska 24, Cracow 31-155, Poland; Photo HiTech Ltd., Bobrzyńskiego 14, Cracow 30-348, Poland.
J Biotechnol. 2024 Nov 20;395:122-140. doi: 10.1016/j.jbiotec.2024.09.017. Epub 2024 Sep 28.
In the present study, we focused on the development and characterization of formulations that function as biological inks. These inks were doped with coumarin derivatives to act as molecular luminescent sensors that allow the monitoring of the kinetics of in situ photopolymerization in 3D (DLP) printing and bioprinting using pneumatic extrusion techniques, making it possible to study the changes in the system in real time. The efficiency of the systems was tested on compositions containing monomers: poly(ethylene glycol) diacrylates and photoinitiators: 2,4,6-trimethylbenzoyldi-phenylphosphinate and lithium phenyl-2,4,6-trimethylbenzoylphosphinate. The selected formulations were spectroscopically characterized and examined for their photopolymerization kinetics and rheological properties. This is important because of the fact that spectroscopic characterization, examination of photopolymerization kinetics, and rheological properties provide valuable insights into the behaviour of photocurable resin dedicated for 3D printing processes. The next step involved printing tests on commercially available 3D printers. In turn, printing carried out as part of the work on commercially available 3D printers further verified the effectiveness of the formulations. Moreover the formulation components and the resulting 3D objects were tested for their antiproliferative effects on the selected Chinese hamster ovary cell line, CHO-K1.
在本研究中，我们专注于开发和表征可作为生物墨水的制剂。这些墨水掺杂了香豆素衍生物，用作分子发光传感器，可监测 3D（DLP）打印和气动挤出技术的生物打印中原位光聚合的动力学，从而能够实时研究系统的变化。在含有单体：聚乙二醇二丙烯酸酯和光引发剂：2,4,6-三甲基苯甲酰基二苯基膦酸酯和苯基-2,4,6-三甲基苯甲酰基膦酸锂的组合物上测试了系统的效率。选择的制剂进行了光谱特性表征，并检查了它们的光聚合动力学和流变性能。这很重要，因为光谱特性表征、光聚合动力学检查和流变性能提供了对专用于 3D 打印过程的可光固化树脂的行为的有价值的见解。下一步是在市售 3D 打印机上进行打印测试。反过来，作为在市售 3D 打印机上进行的工作的一部分进行的打印进一步验证了制剂的有效性。此外，还测试了制剂成分和由此产生的 3D 物体对选定的中国仓鼠卵巢细胞系 CHO-K1 的抗增殖作用。
