Listeria monocytogenes in aquatic food products: Spotlight on epidemiological information, bio-based mitigation strategies and predictive approaches.

Listeria monocytogenes is the foodborne pathogen responsible for listeriosis in humans. Its ability to grow at refrigeration temperatures, particularly in products that support its growth and have a long-refrigerated shelf-life, poses a significant health risk, especially for vulnerable consumer groups such as pregnant women and immunocompromised individuals. A comprehensive analysis of L. monocytogenes in aquatic food products (AFPs) was conducted, examining the prevalence of the bacterium, the associated outbreaks, and the resulting deaths. Data from 66 studies, comprising a total of 19,373 samples, were analysed from the scientific literature to determine prevalence of the pathogen. The mean pooled prevalence of L. monocytogenes was 11 % (95 % CI: 8-14 %) among different AFPs categories. An overview of worldwide listeriosis outbreaks associated with contaminated AFPs between 1980 and 2023 was provided, totalling 1824 cases, including 41 deaths. Furthermore, a compilation of bio-based mitigation strategies was presented, including the use of lactic acid bacteria (LAB) and bacteriophages as bio-protective cultures to inhibit L. monocytogenes in AFPs. A variety of predictive microbiology models, based on growth prediction and interaction for L. monocytogenes, were reviewed to assess the effectiveness of control strategies in different types of AFPs, offering insights into pathogen behaviour throughout the production chain. The reported growth models describe primarily the impact of storage temperature on pathogen growth parameters, while interaction models, which reflect the inhibitory effect of LAB against L. monocytogenes, were generally defined using the Jameson-effect approach and Lotka-Volterra models' family (i.e., predator-prey models). Both models can be used to describe the simultaneous growth of two bacterial populations and their interactions (i.e., amensalism and antagonisms). Several Quantitative Risk assessment studies have been conducted for AFP, identifying the food category as a relevant contributor to Listeriosis risk, and providing predictive insight critical influence of storage temperature, food microbiota, product shelf-life, and population aging on the risk posed by L. monocytogenes. More importantly, this quantitative approach can serve as a key tool to assess the effectiveness of specific mitigation and intervention strategies to control the pathogen, such as sampling schemes or bio-preservation techniques.