Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Int J Biol Macromol. 2024 Nov;281(Pt 2):135708. doi: 10.1016/j.ijbiomac.2024.135708. Epub 2024 Sep 30.
The treatment of diabetic foot ulcers remains a significant challenge, as their morbidity is increasing while current therapies are expensive and often ineffective. The dried mucus from the snail Achatina fulica promotes diabetic wound healing. Herein, to develop a more controllable and stable wound dressing for diabetic wound treatment, the AFG/StarPEG hydrogel mimicking snail mucus was prepared by covalently coupling of sulfated glycosaminoglycan from A. fulica (AFG) with star-shaped polyethylene glycol (StarPEG) amine. The AFG/StarPEG hydrogel reduced excessive inflammation in wound tissues by decreasing pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and increasing anti-inflammatory cytokines (IL-4 and IL-10). Moreover, it promoted the polarization of macrophages to M2 anti-inflammatory type in diabetic wound. By improving transition of diabetic chronic wound from inflammatory phase to proliferative phase, it promoted angiogenesis, collagen deposition and re-epithelialization, and thus tissue regeneration for wound healing. This work provides a convenient and effective dressing for treating chronic diabetic wound.
糖尿病足溃疡的治疗仍然是一个重大挑战，因为其发病率在增加，而目前的治疗方法昂贵且往往无效。非洲大蜗牛的干燥黏液可促进糖尿病伤口愈合。在此，为了开发更可控、更稳定的用于糖尿病伤口治疗的伤口敷料，通过将来自非洲大蜗牛的硫酸化糖胺聚糖（AFG）与星形聚乙二醇（StarPEG）胺共价偶联，制备了模拟蜗牛黏液的 AFG/StarPEG 水凝胶。AFG/StarPEG 水凝胶通过减少促炎细胞因子（IL-6、IL-1β 和 TNF-α）和增加抗炎细胞因子（IL-4 和 IL-10）来减少伤口组织中的过度炎症。此外，它还促进了糖尿病伤口中巨噬细胞向抗炎 M2 型的极化。通过改善糖尿病慢性伤口从炎症期向增生期的转变，促进了血管生成、胶原蛋白沉积和再上皮化，从而促进了伤口愈合的组织再生。这项工作为治疗慢性糖尿病伤口提供了一种方便有效的敷料。
