Ziegler-Skylakakis K, Rossberger S, Andrae U
Arch Toxicol. 1985 Oct;58(1):5-9. doi: 10.1007/BF00292608.
Thiourea was investigated for its capacity to cause DNA alterations in cultured mammalian cells. The induction of DNA repair in primary rat hepatocyte cultures and of gene mutations in V79 Chinese hamster cells were used as biological endpoints. In hepatocytes, thiourea elicited a linear increase in DNA repair replication in the concentration range tested (5-25 mM). In V79 cells, thiourea (10-40 mM) significantly increased the frequency of 8-azaguanine-resistant mutants. The present results show that thiourea is weakly, but definitely, genotoxic and mutagenic in cultured mammalian cells.
研究了硫脲在培养的哺乳动物细胞中引起DNA改变的能力。将原代大鼠肝细胞培养物中DNA修复的诱导以及V79中国仓鼠细胞中的基因突变作为生物学终点。在肝细胞中，在所测试的浓度范围（5-25 mM）内，硫脲引起DNA修复复制呈线性增加。在V79细胞中，硫脲（10-40 mM）显著增加了抗8-氮杂鸟嘌呤突变体的频率。目前的结果表明，硫脲在培养的哺乳动物细胞中具有弱但肯定的遗传毒性和致突变性。
