Wang Guo-Li, Xu Yan-Ling, Zhao Ke-Ming, Sui Ai-Feng, Wang Li-Na, Deng Hu, Wang Ge
First Department of Respiratory and Critical Care Medicine/Pulmonary Disease, The First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110000, Liaoning Province, China.
Second Department of Respiratory and Critical Care Medicine/Pulmonary Disease, The First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110000, Liaoning Province, China.
World J Clin Oncol. 2024 Sep 24;15(9):1198-1206. doi: 10.5306/wjco.v15.i9.1198.
Lung cancer (LC) combined with chronic obstructive pulmonary disease (COPD) is a common combination of comorbidities. Anti-inflammation and modulation of oxidative/antioxidative imbalance may prevent COPD-induced LC, and are also crucial to the treatment of LC combined with COPD. Modern studies have shown that Tao Hong Si Wu Tang (THSW) has vasodilatory, anti-inflammatory, anti-fatigue, anti-shock, immunoregulatory, lipid-reducing, micronutrient-supplementing, and anti-allergy effects.
To observe the effects of THSW on COPD and LC in mice.
A total of 100 specific pathogen-free C57/BL6 mice were randomly divided into five groups: Blank control group (group A), model control group (group B), THSW group (group C), IL-6 group (group D), and THSW + IL-6 group (group E), with 20 mice in each group. A COPD mouse model was established using fumigation plus lipopolysaccharide intra-airway drip, and an LC model was replicated by inoculation using the Lewis cell method.
The blank control group exhibited a clear alveolar structure. The model control and IL-6 groups had thickened alveolar walls, with smaller alveolar lumens, interstitial edema, and several inflammatory infiltrating cells. Histopathological changes in the lungs of the THSW and THSW + IL-6 groups were less than those of the model control group. The serum IL-1β, IL-6, and TNF-α levels and IL-6R, JAK, p-JAK, STAT1/3, p-STAT1/3, FOXO, p-FOXO, and IL-7R expression levels in lung tissues of mice in the rest of the groups were significantly higher than those of the blank control group ( < 0.01). Compared with the model control group, the IL-6 group demonstrated significantly higher levels for the abovementioned proteins in the serum and lung tissues ( < 0.01), and the THSW group had significantly higher serum IL-1β, IL-6, and TNF-α levels and IL-7R expression levels in lung tissues ( < 0.01) but significantly decreased IL-6R, JAK, p-JAK, STAT1/3, p-STAT1/3, FOXO, p-FOXO, and IL-7R levels ( < 0.01).
THSW reduces the serum IL-1β, IL-6, and TNF-α levels in the mouse model with anti-inflammatory effects. Its anti-inflammatory mechanism lies in inhibiting the overactivation of the JAK/STAT1/3 signaling pathway.
肺癌（LC）合并慢性阻塞性肺疾病（COPD）是常见的共病组合。抗炎及调节氧化/抗氧化失衡可能预防COPD诱发的LC，对LC合并COPD的治疗也至关重要。现代研究表明，桃红四物汤（THSW）具有血管舒张、抗炎、抗疲劳、抗休克、免疫调节、降脂、补充微量营养素及抗过敏作用。
观察THSW对小鼠COPD和LC的影响。
将100只无特定病原体的C57/BL6小鼠随机分为五组：空白对照组（A组）、模型对照组（B组）、THSW组（C组）、IL-6组（D组）和THSW + IL-6组（E组），每组20只。采用烟熏加气道内滴注脂多糖建立COPD小鼠模型，用Lewis细胞法接种复制LC模型。
空白对照组肺泡结构清晰。模型对照组和IL-6组肺泡壁增厚，肺泡腔变小，间质水肿，有多处炎性浸润细胞。THSW组和THSW + IL-6组小鼠肺组织的组织病理学变化小于模型对照组。其余各组小鼠血清IL-1β、IL-6和TNF-α水平以及肺组织中IL-6R、JAK、p-JAK、STAT1/3、p-STAT1/3、FOXO、p-FOXO和IL-7R表达水平均显著高于空白对照组（P < 0.01）。与模型对照组相比，IL-6组血清和肺组织中上述蛋白水平显著更高（P < 0.01），THSW组血清IL-1β、IL-6和TNF-α水平以及肺组织中IL-7R表达水平显著更高（P < 0.01），但IL-6R、JAK、p-JAK、STAT1/3、p-STAT1/3、FOXO、p-FOXO和IL-7R水平显著降低（P < 0.01）。
THSW降低小鼠模型血清IL-1β、IL-6和TNF-α水平，具有抗炎作用。其抗炎机制在于抑制JAK/STAT1/3信号通路的过度激活。
