Kanaeva I P, Skotselias E D, Kuznetsova G P, Antonova G N, Bachmanova G I
Biokhimiia. 1985 Aug;50(8):1382-8.
Emulgen 913, Triton N-101 and sodium cholate were compared for their reconstituting action on the dimethylaniline N-demethylation system containing cytochrome P-450 and NADPH-cytochrome P-450 reductase. The comparison showed that emulgen 913 is the most efficient detergent. The optimum molar ratio of the proteins and emulgen appeared to be equal to 1:1:600. Study on the mechanism of emulgen reconstituting action showed that this effect is due to the mixed complex formation between the cytochrome and reductase, the complexes containing five molecules of the flavoprotein and five molecules of cytochrome P-450. No formation of mixed protein aggregates or reconstitution was observed in the absence of the detergent or at its concentrations exceeding the optimum level.
比较了乳化剂913、曲拉通N - 101和胆酸钠对含有细胞色素P - 450和NADPH - 细胞色素P - 450还原酶的二甲基苯胺N - 脱甲基化系统的重组作用。比较结果表明，乳化剂913是最有效的去污剂。蛋白质与乳化剂的最佳摩尔比似乎等于1:1:600。对乳化剂重组作用机制的研究表明，这种作用是由于细胞色素和还原酶之间形成了混合复合物，该复合物含有五个黄素蛋白分子和五个细胞色素P - 450分子。在没有去污剂或去污剂浓度超过最佳水平时，未观察到混合蛋白聚集体的形成或重组。
