Jose Jismon, Amiben Lakhani, Girish B P, Sen Kakali, Prasad T N V K V, Roy Sujit, Roy Choudhury Swarup
Department of Biology, Indian Institute of Science Education and Research, Tirupati, India.
Regional Agricultural Research Station, Institute of Frontier Technology, Acharya N G Ranga Agricultural University, Tirupati, India.
Plant Cell Environ. 2025 Jan;48(1):818-837. doi: 10.1111/pce.15179. Epub 2024 Oct 1.
Desiccation tolerance is a complex biological phenomenon that allows certain plants to survive extreme dehydration and revive upon rehydration. Although significant progress has been made in understanding the physiological and molecular mechanisms involved in desiccation tolerance, recovery mechanisms after prolonged desiccation periods are enigmatic. Combining physiological, biochemical, transcriptomic and metabolomic approaches, we investigated the role of prolonged desiccation on recovery of Selaginella bryopteris. Prolonged desiccation causes a decline in the antioxidant system, leading to accumulation of ROS that hinder recovery by inducing cellular damage. Transcriptome and WGCNA analysis revealed the significance of protective proteins, alternative respiration and protein homeostasis in cellular protection and recovery after short and long-term desiccation. Metabolomic analysis exhibited an increased accumulation of antioxidant compounds, which can be substituted for antioxidant enzymes to maintain cellular protection during prolonged desiccation. The significant role of autophagy and autophagic components was evaluated by HO treatment and phylogenetic analysis of ATG4 and ATG8, which unveiled their substantial role in desiccation tolerance and remarkable conservation of the autophagy-related genes across plant species. Our data demonstrated that prolonged desiccation leads to ROS-induced cell death by extensive autophagy due to enormous loss of protective proteins, antioxidant enzymes and energy resources during desiccation.
耐旱性是一种复杂的生物学现象，它使某些植物能够在极端脱水的情况下存活，并在重新水化后复苏。尽管在理解耐旱性所涉及的生理和分子机制方面已经取得了重大进展，但长时间脱水后的恢复机制仍然是个谜。我们结合生理、生化、转录组学和代谢组学方法，研究了长时间脱水对卷柏恢复的作用。长时间脱水会导致抗氧化系统下降，导致活性氧积累，通过诱导细胞损伤来阻碍恢复。转录组和加权基因共表达网络分析（WGCNA）揭示了保护蛋白、交替呼吸和蛋白质稳态在短期和长期脱水后的细胞保护和恢复中的重要性。代谢组学分析显示抗氧化化合物积累增加，这些化合物可以替代抗氧化酶，在长时间脱水期间维持细胞保护。通过3-甲基腺嘌呤（3-MA）处理以及对自噬相关基因ATG4和ATG8的系统发育分析，评估了自噬和自噬成分的重要作用，这揭示了它们在耐旱性中的重要作用以及自噬相关基因在植物物种间的显著保守性。我们的数据表明，长时间脱水会由于脱水期间保护蛋白、抗氧化酶和能量资源的大量损失，通过广泛的自噬导致活性氧诱导的细胞死亡。
