Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Center for Biosystems Dynamics Research, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan.
Int J Syst Evol Microbiol. 2024 Oct;74(10). doi: 10.1099/ijsem.0.006537.
Two strains, designated JCM 36746 and JCM 36749, were isolated from Bengal clock vine () and soil, respectively, in Okinawa, Japan. Analysis of the internal transcribed spacer (ITS) regions and D1/D2 domains of the large subunit rRNA gene sequences revealed identical sequences in both strains, indicating that they belong to the same species. Sequence analysis and physiological characterization identified these strains as representing a novel yeast species in the genus . The sequence similarities of the concatenated ITS regions and D1/D2 domains indicated that JCM 36746 and JCM 36749 formed a well-supported distinct from closely related species belonging to the clade, including , , , , , , , , , , , , , and three undescribed species, comprising aff. / Y151, sp. GE19S08, and sp. strain NYNU 22830. The sequences of the D1/D2 domains and ITS regions differed in nucleotide substitutions by 1.51% and 2.57% or greater, respectively, from those of the previously described and undescribed related species. In addition, the physiological characteristics of the novel species were distinct from those of the closely related described species. On the basis of these findings, we propose the name sp. nov. to classify this species within the genus . The holotype used is JCM 36746 (ex-type strains CBS 18614 and NBRC 116657). The MycoBank accession number is MB 853823.
两株菌，分别命名为 JCM 36746 和 JCM 36749，从日本冲绳的孟加拉时钟藤（）和土壤中分离得到。对内转录间隔区（ITS）和大亚基 rRNA 基因序列的 D1/D2 结构域的分析表明，两株菌的序列完全相同，表明它们属于同一物种。序列分析和生理特性鉴定表明，这些菌株代表了属中的一个新的酵母物种。ITS 区和 D1/D2 区的序列相似性表明，JCM 36746 和 JCM 36749 与亲缘关系密切的种形成了一个良好支持的分支，包括,,,,,,,,,,, 和三个未描述的种，包括 aff. / Y151, sp. GE19S08 和 sp. strain NYNU 22830。D1/D2 区和 ITS 区的序列在核苷酸取代方面分别与先前描述的和未描述的相关种相差 1.51%和 2.57%或更多。此外，新种的生理特性与亲缘关系密切的描述种不同。基于这些发现，我们提出了 sp. nov. 来分类该物种在属内。使用的模式菌株是 JCM 36746（CBS 18614 和 NBRC 116657 的 ex-type 菌株）。MycoBank 登录号为 MB 853823。
