Zhang Yichi, Liu Xinyu, Wang Junyan, Wang Ying, Amponsah Priscilla, Tang Tao, Jones Andrew K, Zhao Chunqing
Key Laboratory of Integrated Pest Management on Crops in East China, Ministry of Agriculture and Rural Affairs/Key Laboratory of Integrated Management of Crop Diseases and Pests, Education of Ministry, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P. R. China.
Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, P. R. China.
J Agric Food Chem. 2024 Oct 1. doi: 10.1021/acs.jafc.4c06478.
Fluralaner is a novel insecticide targeting the ionotropic GABA receptor (GABAR) subunit, RDL. A recent study revealed that N316L, a substitution of asparagine (N) with leucine (L), in the second transmembrane (M2)-spanning region reduced the antagonist action of fluralaner on the housefly RDL (RDL) . To verify the impact of N316L , the corresponding mutation (N318L) in the fruitfly RDL (RDL) was constructed using CRISPR/Cas9 genome editing. The homozygous RDL mutant showed a 9.87-fold resistance to fluralaner compared with while still being highly sensitive to broflanilide and fipronil, which is consistent with those findings observed in the electrophysiology assays of the homomeric RDL or RDL channel. Moreover, RDL led to malformed ovaries, stunted eggs, and sterility in homozygous females. These results highlighted N318 as a molecular site for fluralaner and and might elucidate the resistance mechanisms of insects against fluralaner.
氟虫腈是一种新型杀虫剂，作用于离子型γ-氨基丁酸受体（GABAR）亚基RDL。最近的一项研究表明，在第二个跨膜（M2）区域，天冬酰胺（N）被亮氨酸（L）取代形成的N316L突变，降低了氟虫腈对家蝇RDL的拮抗作用。为了验证N316L的影响，利用CRISPR/Cas9基因组编辑技术在果蝇RDL中构建了相应的突变（N318L）。与野生型相比，纯合RDL突变体对氟虫腈具有9.87倍的抗性，同时对溴虫氟苯双酰胺和氟虫腈仍高度敏感，这与在同型RDL或RDL通道的电生理试验中观察到的结果一致。此外，RDL突变导致纯合雌性果蝇卵巢畸形、卵发育不良和不育。这些结果突出了N318作为氟虫腈作用的分子位点，可能阐明昆虫对氟虫腈的抗性机制。
