Lu Louisa, Fauci Ali, Richardson Quintin R, Mahmud Hamidah, Nesemann John M, Aragie Solomon, Hailu Dagnachew, Dagnew Adane, Chernet Ambahun, Tadesse Zerihun, Zeru Taye, Thompson Isabel J B, Wittberg Dionna M, Nash Scott D, Lietman Thomas M, Keenan Jeremy D
Francis I. Proctor Foundation, University of California, San Francisco, California.
Department of Ophthalmology, Stanford University, Stanford, California.
Am J Trop Med Hyg. 2024 Oct 1;111(6):1256-1258. doi: 10.4269/ajtmh.24-0319. Print 2024 Dec 4.
The purpose of this study was to investigate the correlation between bacterial load of Chlamydia trachomatis as measured from quantitative polymerase chain reaction (qPCR) and the relative clinical severity of trachomatous inflammation. Individuals with trachoma from rural communities in Ethiopia had photographs taken as well as swabs obtained of the upper tarsal conjunctivas. Conjunctival swabs were processed with PCR assay, which provided quantitative results of ocular chlamydial load. A series of 125 conjunctival photographs were ranked from least to most severe according to clinical severity for follicular and papillary conjunctivitis. Higher intensity rankings of trachomatous inflammation were associated with higher chlamydial load for both follicular inflammation (Spearman's ρ = 0.43; P <0.001) and papillary inflammation (Spearman's ρ = 0.50; P <0.001). Rankings of trachomatous inflammation may be a clinically meaningful indicator of trachoma.
本研究的目的是调查通过定量聚合酶链反应（qPCR）测量的沙眼衣原体细菌载量与沙眼性炎症相对临床严重程度之间的相关性。来自埃塞俄比亚农村社区的沙眼患者拍摄了照片，并获取了上睑结膜拭子。结膜拭子通过PCR检测进行处理，该检测提供了眼部衣原体载量的定量结果。根据滤泡性和乳头性结膜炎的临床严重程度，对一系列125张结膜照片从最轻到最严重进行了排名。对于滤泡性炎症（Spearman等级相关系数ρ = 0.43；P <0.001）和乳头性炎症（Spearman等级相关系数ρ = 0.50；P <0.001），沙眼性炎症的强度排名越高，衣原体载量越高。沙眼性炎症的排名可能是沙眼具有临床意义的指标。
