Sandy C M, Guimarães C C, Fonseca V M B, Nicoli J R, Martins F S, Borges M C
Department of Internal Medicine, Ribeirao Preto Medical School, 54539University of São Paulo, São Paulo, SP, Brazil.
Department of Microbiology, Institute of Biological Sciences, 113014Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Benef Microbes. 2024 Sep 27;16(1):125-134. doi: 10.1163/18762891-bja00044.
Asthma prevalence has been increasing in communities that become more urbanised. Our previous results showed that Saccharomyces cerevisiae UFMG A-905 prevented the development of asthma symptoms and characteristics in a dose-dependent manner. Perinatal programming theory proposes that early exposure to some stimuli may have a protective effect in adult life. The aim of this study was to evaluate the effects of perinatal administration of S. cerevisiae UFMG A-905 in the prevention of asthma in the offspring of mice. S. cerevisiae UFMG A-905 was cultured in YPD broth medium and administered to three groups of mice: before conception, during gestation and lactation (CGL group); during gestation and lactation (GL group); and only during lactation (L group). The offspring of these animals were sensitised and challenged with ovalbumin. Two control groups received saline in the same periods. After, in vivo measurements of airway hyperresponsiveness (AHR) were performed. Total and differential cell count in bronchoalveolar lavage (BAL); ELISA for interleukin (IL)-4, IL-5, IL-10, IL-13, and IL-17A in the lung homogenate or BAL; and ELISA for ovalbumin (OVA)-specific immunoglobulin E (IgE) were performed. The animals of the CGL, GL, and L group, when compared to the OVA group, presented a significant reduction of AHR ( P < 0.01), levels of IL-5 ( P < 0.001) in BAL, and IL-4 ( P < 0.05) and IL-13 ( P < 0.01) in the lung homogenate. Serum IgE levels were significantly higher ( P < 0.05) in CGL and GL groups when compared to the OVA group, but not in the L group. Only in the group L, there was a significant decrease in the number of total cells ( P < 0.01) and eosinophils ( P < 0.05). Perinatal administration of S. cerevisiae UFMG A-905 prevented the development of asthma-like characteristics and may be an option for asthma management. The protective effects on the offspring were more prominent when the yeast was given during lactation.
在变得更加城市化的社区中，哮喘患病率一直在上升。我们之前的研究结果表明，酿酒酵母UFMG A - 905以剂量依赖的方式预防哮喘症状和特征的发展。围产期编程理论提出，早期接触某些刺激可能对成年生活具有保护作用。本研究的目的是评估围产期给予酿酒酵母UFMG A - 905对预防小鼠后代哮喘的影响。酿酒酵母UFMG A - 905在YPD肉汤培养基中培养，并给予三组小鼠：受孕前、妊娠期和哺乳期（CGL组）；妊娠期和哺乳期（GL组）；以及仅在哺乳期（L组）。这些动物的后代用卵清蛋白进行致敏和激发。两个对照组在相同时间段接受生理盐水。之后，进行气道高反应性（AHR）的体内测量。进行支气管肺泡灌洗（BAL）中的总细胞和分类细胞计数；检测肺匀浆或BAL中白细胞介素（IL）-4、IL - 5、IL - 10、IL - 13和IL - 17A的酶联免疫吸附测定（ELISA）；以及检测卵清蛋白（OVA）特异性免疫球蛋白E（IgE）的ELISA。与OVA组相比，CGL组、GL组和L组的动物AHR显著降低（P < 0.01），BAL中IL - 5水平显著降低（P < 0.001），肺匀浆中IL - 4（P < 0.05）和IL - 13（P < 0.01）水平显著降低。与OVA组相比，CGL组和GL组的血清IgE水平显著更高（P < 0.05），但L组并非如此。仅在L组中，总细胞数量（P < 0.01）和嗜酸性粒细胞数量（P < 0.05）显著减少。围产期给予酿酒酵母UFMG A - 905可预防哮喘样特征的发展，可能是哮喘管理的一种选择。当在哺乳期给予酵母时，对后代的保护作用更为显著。
