Fanzor2的结构揭示了对TnpB超家族进化的见解。
Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily.
作者信息
Schargel Richard D, Qayyum M Zuhaib, Tanwar Ajay Singh, Kalathur Ravi C, Kellogg Elizabeth H
机构信息
Department of Microbiology, Cornell University, Ithaca, NY, USA.
Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
出版信息
Nat Struct Mol Biol. 2025 Feb;32(2):243-246. doi: 10.1038/s41594-024-01394-4. Epub 2024 Oct 1.
RNA-guided endonucleases, once thought to be exclusive to prokaryotes, have been recently identified in eukaryotes and are called Fanzors. They are classified into two clades, Fanzor1 and Fanzor2. Here we present the cryo-electron microscopy structure of Acanthamoeba polyphaga mimivirus Fanzor2, revealing its ωRNA architecture, active site and features involved in transposon-adjacent motif recognition. A comparison to Fanzor1 and TnpB structures highlights divergent evolutionary paths, advancing our understanding of RNA-guided endonucleases.
RNA引导的核酸内切酶曾被认为是原核生物所特有的，最近在真核生物中也被发现，被称为Fanzors。它们被分为两个进化枝，Fanzor1和Fanzor2。在这里，我们展示了多噬棘阿米巴拟病毒Fanzor2的冷冻电子显微镜结构，揭示了其ωRNA结构、活性位点以及参与转座子相邻基序识别的特征。与Fanzor1和TnpB结构的比较突出了不同的进化路径，增进了我们对RNA引导的核酸内切酶的理解。
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