Ono K, Kasuya Y, Shigenobu K
Eur J Pharmacol. 1985 Nov 5;117(2):159-68. doi: 10.1016/0014-2999(85)90600-4.
To investigate whether organ culture is a suitable in vitro model for studying the mechanisms of denervation-induced supersensitivity, we cultured 1-week-old rat vas deferens for 3 days with a basic applied tension of 20 mg. Cultured muscles showed supersensitivity to norepinephrine and methacholine with concomitant elevation of the maximal response. To compare these changes with those caused by denervation, young rats were chemically denervated by injecting 6-hydroxydopamine, and consequent sensitivity changes were investigated. Denervated muscles showed non-specific supersensitivity to norepinephrine and methacholine but the maximal response did not increase. When these denervated muscles were organ-cultured, they showed no or only a slight increase in sensitivity to norepinephrine and methacholine, but the maximal response increased greatly. These observations led to the suggestion that the increase in sensitivity may be mediated through the same mechanisms as those for denervation supersensitivity. The elevation of the maximal response was suggested to be produced by the improvement of cell-to-cell conduction as well as some other unknown factor(s) probably specific to organ culture. Thus, it was concluded that organ-cultured 1-week-old rat vas deferens is a useful model to study the mechanisms of denervation supersensitivity.
为了研究器官培养是否是一种适合用于研究去神经支配诱导的超敏反应机制的体外模型，我们将1周龄大鼠的输精管以20mg的基础施加张力培养3天。培养的肌肉对去甲肾上腺素和乙酰甲胆碱表现出超敏反应，同时最大反应升高。为了将这些变化与去神经支配引起的变化进行比较，通过注射6-羟基多巴胺对幼鼠进行化学去神经支配，并研究随后的敏感性变化。去神经支配的肌肉对去甲肾上腺素和乙酰甲胆碱表现出非特异性超敏反应，但最大反应并未增加。当这些去神经支配的肌肉进行器官培养时，它们对去甲肾上腺素和乙酰甲胆碱的敏感性没有增加或仅略有增加，但最大反应大幅增加。这些观察结果表明，敏感性的增加可能是通过与去神经支配超敏反应相同的机制介导的。最大反应的升高被认为是由细胞间传导的改善以及可能特定于器官培养的一些其他未知因素引起的。因此，得出结论，器官培养的1周龄大鼠输精管是研究去神经支配超敏反应机制的有用模型。
