Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Recent Adv Drug Deliv Formul. 2024;18(4):304-314. doi: 10.2174/0126673878297658240804192222.
Autophagy plays a crucial role in modulating the proliferation of cancer diseases. However, the application of Naringenin (Nar), a compound with potential benefits against these diseases, has been limited due to its poor solubility and bioavailability.
This study aimed to develop solid lipid nanoparticles (Nar-SLNs) loaded with Nar to enhance their therapeutic impact.
experiments using Rin-5F cells exposed to Nar and Nar-SLNs were carried out to investigate the protective effects of Nar and its nanoformulation against the pancreatic cancer cell line of Rin-5F.
Treatment with Nar and Nar-SLN led to an increase in autophagic markers (Akt, LC3, Beclin1, and ATG genes) and a decrease in the level of miR-21. Both Nar and Nar-SLN treatments inhibited cell proliferation and reduced the expression of autophagic markers. Notably, Nar-SLNs exhibited greater efficacy compared to free Nar.
These findings suggest that SLNs effectively enhance the cytotoxic impact of Nar, making Nar-SLNs a promising candidate for suppressing or preventing Rin-5F cell growth.
自噬在调节癌症疾病的增殖方面起着至关重要的作用。然而，由于其溶解度和生物利用度差，具有潜在益处的柚皮素（Nar）化合物的应用受到限制。
本研究旨在开发负载柚皮素的固体脂质纳米粒（Nar-SLNs），以增强其治疗效果。
用柚皮素和 Nar-SLNs 处理 Rin-5F 细胞，研究柚皮素及其纳米制剂对 Rin-5F 胰腺癌细胞系的保护作用。
柚皮素和 Nar-SLN 处理导致自噬标志物（Akt、LC3、Beclin1 和 ATG 基因）增加，miR-21 水平降低。柚皮素和 Nar-SLN 处理均抑制细胞增殖并降低自噬标志物的表达。值得注意的是，Nar-SLNs 的效果优于游离柚皮素。
这些发现表明 SLNs 可有效增强 Nar 的细胞毒性作用，使 Nar-SLNs 成为抑制或预防 Rin-5F 细胞生长的有前途的候选物。
