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仓鼠肺泡巨噬细胞表面的碳水化合物：一项使用荧光素偶联凝集素的研究。

The carbohydrates associated with the surfaces of hamster alveolar macrophages: a study using fluorescein-conjugated lectins.

作者信息

Meban C

出版信息

J Anat. 1985 Mar;140 ( Pt 2)(Pt 2):221-8.

PMID:3935630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165160/
Abstract

Alveolar macrophages were isolated from the lungs of hamsters by lavage and centrifugation. The harvesting procedure yielded about 5.2 X 10(6) cells per animal and the majority of these cells were viable as assessed by a Trypan blue exclusion test. Monolayers of macrophages were labelled with a series of FITC-conjugated lectins. The results of this study suggest that the surface coating of the alveolar macrophages contains the following carbohydrate groups: N-acetylgalactosamine, N-acetylglucosamine, D-glucose, D-mannose, L-fucose and sialic acid.

摘要

通过灌洗和离心从仓鼠肺中分离出肺泡巨噬细胞。收获过程中每只动物获得约5.2×10⁶个细胞，通过台盼蓝排斥试验评估，这些细胞中的大多数是活细胞。用一系列异硫氰酸荧光素（FITC）偶联的凝集素标记巨噬细胞单层。本研究结果表明，肺泡巨噬细胞的表面包被含有以下碳水化合物基团：N-乙酰半乳糖胺、N-乙酰葡糖胺、D-葡萄糖、D-甘露糖、L-岩藻糖和唾液酸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/a84b2789a69f/janat00194-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/e26a8a9bad1f/janat00194-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/0ad2a7c459d9/janat00194-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/a974ead05c44/janat00194-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/77d8e1ce55df/janat00194-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/a84b2789a69f/janat00194-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/e26a8a9bad1f/janat00194-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/0ad2a7c459d9/janat00194-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/a974ead05c44/janat00194-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/77d8e1ce55df/janat00194-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04dd/1165160/a84b2789a69f/janat00194-0044-b.jpg

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引用本文的文献

1
Ultrastructural visualisation of carbohydrate groups in the surface coating of hamster alveolar macrophages and pneumonocytes.仓鼠肺泡巨噬细胞和肺细胞表面被覆物中碳水化合物基团的超微结构观察
J Anat. 1986 Jun;146:131-9.

本文引用的文献

1
THE PARTICULATE HYDROLASES OF MACROPHAGES. I. COMPARATIVE ENZYMOLOGY, ISOLATION, AND PROPERTIES.巨噬细胞的颗粒水解酶。I. 比较酶学、分离及特性
J Exp Med. 1963 Dec 1;118(6):991-1008. doi: 10.1084/jem.118.6.991.
2
THE PARTICULATE HYDROLASES OF MACROPHAGES. II. BIOCHEMICAL AND MORPHOLOGICAL RESPONSE TO PARTICLE INGESTION.巨噬细胞的颗粒水解酶。II. 对颗粒摄取的生化和形态学反应。
J Exp Med. 1963 Dec 1;118(6):1009-20. doi: 10.1084/jem.118.6.1009.
3
A histochemical study of phagocytic and enzymatic functions of rabbit mononuclear and polymorphonuclear exudate cells and alveolar macrophages. I. Survey and quantitation of enzymes, and states of cellular activation.
兔单核及多形核渗出细胞和肺泡巨噬细胞吞噬及酶功能的组织化学研究。I. 酶的检测与定量以及细胞活化状态
J Cell Biol. 1963 Jun;17(3):465-86. doi: 10.1083/jcb.17.3.465.
4
Lysozyme content of alveolar and peritoneal macrophages from the rabbit.兔肺泡巨噬细胞和腹腔巨噬细胞的溶菌酶含量
J Immunol. 1961 Feb;86:133-6.
5
Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins.十二种凝集素对与N-糖基化蛋白相关的寡糖和糖肽的特异性。
Eur J Biochem. 1981 Jun;117(1):41-55. doi: 10.1111/j.1432-1033.1981.tb06300.x.
6
Role of alveolar macrophage plasminogen activator in the acute pulmonary responses to endotoxin.肺泡巨噬细胞纤溶酶原激活剂在内毒素所致急性肺反应中的作用
Lung. 1984;162(1):49-58. doi: 10.1007/BF02715627.
7
Beta-D-xylosidase in pig kidney.猪肾中的β-D-木糖苷酶
Biochim Biophys Acta. 1967 Jan 11;132(1):212-4. doi: 10.1016/0005-2744(67)90215-x.
8
Some physical and chemical properties of concanavalin A, the phytohemagglutinin of the jack bean.刀豆球蛋白A（刀豆的植物血凝素）的一些物理和化学性质。
Biochemistry. 1967 Jan;6(1):105-11. doi: 10.1021/bi00853a018.
9
Macrophage plasma membrane. II. Studies on synthesis and turnover of protein constituents.巨噬细胞质膜。II. 蛋白质成分的合成与周转研究。
J Exp Med. 1971 Apr 1;133(4):807-20. doi: 10.1084/jem.133.4.807.
10
The hyaluronidase of rat skin.大鼠皮肤的透明质酸酶
Arch Biochem Biophys. 1969 Dec;135(1):387-95. doi: 10.1016/0003-9861(69)90554-2.