Pharmacognosy Department, College of Pharmacy, Cairo University, Cairo, Kasr El Aini St., P.B. 11562, Egypt.
Pharmacognosy Department, College of Pharmacy, Egyptian Russian University, Badr City 11829, Cairo, Egypt.
Mar Pollut Bull. 2024 Nov;208:116983. doi: 10.1016/j.marpolbul.2024.116983. Epub 2024 Oct 1.
Recently, the impacts of climate change, notably ocean warming and solar ultraviolet radiation, have led to significant stress and mortality in cnidarians. The objective of this study is to decode the metabolic responses of sea anemones Entacmaea quadricolor and upside-down jellyfish Cassiopea andromeda upon exposure to thermal and ultraviolet stress. Gas chromatography-mass spectrometry and ultraperformance liquid chromatography coupled with high-resolution mass spectrometry targeting polar and non-polar metabolites were applied. In total, 72 polar and 242 lipophilic metabolites were detected in jellyfish and sea anemones, respectively. Amino acids are the major metabolite class in jellyfish, and triacylglycerides are the predominant lipids in jellyfish and anemones. Exposure to stressors led to metabolic alterations, marked by elevated amino acids in jellyfish and increased amino acids and sugar alcohols in sea anemones at 34 °C and after four days of ultraviolet radiation. Non-polar metabolome analysis indicated distinct responses to ultraviolet radiation and thermal stress in both species.
最近，气候变化的影响，特别是海洋变暖与太阳紫外线辐射，导致了刺胞动物（珊瑚、海葵、水母等）的重大压力与死亡率。本研究旨在解码海葵 Entacmaea quadricolor 和倒立水母 Cassiopea andromeda 在暴露于热和紫外线压力下的代谢反应。应用气相色谱-质谱法和超高效液相色谱与高分辨率质谱联用技术，靶向极性和非极性代谢物。总共在水母和海葵中分别检测到 72 种极性和 242 种亲脂性代谢物。在水母中，氨基酸是主要的代谢物类别，而在水母和海葵中，三酰甘油是主要的脂质。暴露于胁迫因子会导致代谢改变，在 34°C 下和紫外线辐射四天后，水母中氨基酸升高，海葵中氨基酸和糖醇增加。非极性代谢组学分析表明，这两个物种对紫外线辐射和热胁迫有明显的反应。
