CUMT-UCASAL Joint Research Center for Biomining and Soil Ecological Restoration, State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology, Xuzhou, Jiangsu province, 221116, China; State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology (Beijing), Beijing, 100083, China.
State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology (Beijing), Beijing, 100083, China.
J Environ Manage. 2024 Nov;370:122708. doi: 10.1016/j.jenvman.2024.122708. Epub 2024 Oct 2.
The harsh climatic conditions and severe scarcity of surface soil present significant challenges to ecological restoration in open-pit mine dumps within China's type II plant cold resistance zone. To address the topsoil shortage, mineral black clay was used to create synthetic soil. This study explored the application of an ecological restoration bacteria (ERB) consortium to accelerate the ecological restoration of synthetic soil-covered areas by enhancing soil ecosystem construction. The results demonstrated that ERB significantly influenced the native bacterial community structure in mixed black clay. Specifically, ERB disrupted the inhibitory effects of the Actinobacterota phylum on the development of native bacterial diversity, leading to an increase in unclassified_o_Solirubrobacterales sp., norank_f_norank_o_norank_c_KD4-96 sp., Sphingomonas sp., Luteitalea sp., norank_f_Vicinamibacteraceae sp., and other aerobic and anaerobic bacteria. These alterations in soil microbial structure directly impacted soil composition and vegetation diversity. The plant diversity survey and metabolomics analysis revealed that the reduction of harmful substances, such as HPED, HODE, and HOME, in black clay soil improved the growth and distribution of Salsola collina Pall. and Medicago sativa L. This increase facilitated the cycling of key nutrients, such as nitrogen (N) and phosphorus (P), and promoted the establishment of symbiotic relationships between plants, microorganisms, and soil. Ultimately, the ecological remediation of the synthetic soil was achieved through the synergistic effects of ERB, which included the degradation of inhibitory soil components, enhanced nutrient consumption by microbiota and plants, and the overall promotion of ecosystem stability in the reclamation area.
在中国 II 型植物抗寒区的露天矿排土场中，恶劣的气候条件和严重缺乏表土对生态恢复构成了重大挑战。为了解决表土短缺问题，采用了矿物黑土来制造人工合成土壤。本研究探讨了应用生态修复细菌（ERB）联合体来通过增强土壤生态系统建设来加速人工合成土壤覆盖区域的生态恢复。研究结果表明，ERB 显著影响了混合黑土中本地细菌群落结构。具体来说，ERB 破坏了放线菌门对本地细菌多样性发展的抑制作用，导致未分类的_o_Solirubrobacterales sp.、norank_f_norank_o_norank_c_KD4-96 sp.、Sphingomonas sp.、Luteitalea sp.、norank_f_Vicinamibacteraceae sp. 和其他需氧和厌氧细菌的增加。这些土壤微生物结构的变化直接影响了土壤组成和植被多样性。植物多样性调查和代谢组学分析表明，黑土中 HPED、HODE 和 HOME 等有害物质的减少改善了猪毛菜和紫花苜蓿的生长和分布。这种增加促进了氮（N）和磷（P）等关键养分的循环，并促进了植物、微生物和土壤之间共生关系的建立。最终，通过 ERB 的协同作用实现了人工合成土壤的生态修复，包括抑制性土壤成分的降解、微生物和植物对养分的消耗增强以及开垦区生态系统稳定性的全面提升。
